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FCM/FACS (Flow Cytometry)
(VHL Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit VHL Polyclonal Antibody | anti-VHL antibody
VHL Antibody (N-term)
Gene Names
VHL; RCA1; VHL1; pVHL; HRCA1
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
VHL, Antibody; VHL Antibody (N-term); Von Hippel-Lindau disease tumor suppressor; Protein G7; pVHL; VHL; anti-VHL antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This VHL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human VHL.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
43-71
Sequence Length
160
Applicable Applications for anti-VHL antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Involved in the ubiquitination and subsequent
proteasomal degradation via the von
Hippel-Lindau ubiquitination complex. Seems
to act as a target recruitment subunit in the
E3 ubiquitin ligase complex and recruits
hydroxylated hypoxia- inducible factor (HIF)
under normoxic conditions. Involved in
transcriptional repression through interaction
with HIF1A, HIF1AN and histone deacetylases.
Ubiquitinates, in an oxygen-responsive
manner, ADRB2.
Cellular Location
Isoform 1: Cytoplasm. Membrane; Peripheral
membrane protein. Nucleus. Note=Found
predominantly in the cytoplasm and with less
amounts nuclear or membrane-associated
Colocalizes with ADRB2 at the cell membrane
Tissue Location
Expressed in the adult and fetal brain and kidney
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(VHL Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(VHL Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of VHL Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of VHL Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemisry)
(VHL Antibody (N-term) (RB18667) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VHL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(VHL Antibody (N-term) (RB18667) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VHL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of VHL antibody (N-term) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of VHL antibody (N-term) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Anti-VHL Antibody (N-term) at 1:2000 dilution + F9 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-VHL Antibody (N-term) at 1:2000 dilution + F9 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-VHL antibody
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome. The protein is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein.
Product Categories/Family for anti-VHL antibody
References
Olmos,G., Cell. Mol. Life Sci. 66 (13), 2167-2180 (2009) Hatzimichael,E., Clin Lymphoma Myeloma 9 (3), 239-242 (2009) Luu,V.D., Clin. Cancer Res. 15 (10), 3297-3304 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24153
NCBI Official Full Name
von Hippel-Lindau disease tumor suppressor isoform 1
NCBI Official Synonym Full Names
von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
NCBI Official Symbol
VHL
NCBI Official Synonym Symbols
RCA1; VHL1; pVHL; HRCA1
NCBI Protein Information
von Hippel-Lindau disease tumor suppressor
UniProt Protein Name
Von Hippel-Lindau disease tumor suppressor
UniProt Gene Name
VHL
UniProt Entry Name
VHL_HUMAN
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Product Notes
The VHL vhl (Catalog #AAA286195) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 43-71. The VHL Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's VHL can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the VHL vhl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VHL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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