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product-image-AAA288188_IHC11.jpg IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma)

Rabbit anti-Human, mouse VILIP3 Polyclonal Antibody | anti-HPCAL1 antibody

VILIP3 Antibody (N-term)

Gene Names
HPCAL1; BDR1; HLP2; VILIP-3
Reactivity
Human, mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
VILIP3, Antibody; VILIP3 Antibody (N-term); Hippocalcin-like protein 1; Calcium-binding protein BDR-1; HLP2; Visinin-like protein 3; VILIP-3; HPCAL1; BDR1; anti-HPCAL1 antibody
Ordering
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This VILIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human VILIP3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
2-32
Sequence Length
193
Applicable Applications for anti-HPCAL1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma)

product-image-AAA288188_IHC11.jpg IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma)

WB (Western Blot)

(The anti-VILIP3 Pab is used in Western blot to detect VILIP3 in mouse cerebellum tissue lysate.)

product-image-AAA288188_WB13.jpg WB (Western Blot) (The anti-VILIP3 Pab is used in Western blot to detect VILIP3 in mouse cerebellum tissue lysate.)

WB (Western Blot)

(Western blot analysis of VILIP3 (arrow) using rabbit polyclonal VILIP3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VILIP3 gene (Lane 2) (Origene Technologies).)

product-image-AAA288188_WB15.jpg WB (Western Blot) (Western blot analysis of VILIP3 (arrow) using rabbit polyclonal VILIP3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VILIP3 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-HPCAL1 antibody
VILIP3 is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. There are two alternatively spliced transcript variants of this gene, with multiple polyadenylation sites. Transcript variant 1 utilizes a different exon and also lacks one exon in the 5' UTR, as compared to variant 2; thus, the encoded protein is the same.
Product Categories/Family for anti-HPCAL1 antibody
References
Braunewell, K., et al., Dement Geriatr Cogn Disord 12(2):110-116 (2001). Bernstein, H.G., et al., J Neurocytol 28(8):655-662 (1999). Kobayashi, M., et al., Biochim. Biophys. Acta 1222(3):515-518 (1994). Hidaka, H., et al., Neurosci. Res. 16(2):73-77 (1993). Ivings, L., et al., Biochem. J. 363 (Pt 3), 599-608 (2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22313
NCBI Official Full Name
hippocalcin-like protein 1
NCBI Official Synonym Full Names
hippocalcin-like 1
NCBI Official Symbol
HPCAL1
NCBI Official Synonym Symbols
BDR1; HLP2; VILIP-3
NCBI Protein Information
hippocalcin-like protein 1
UniProt Protein Name
Hippocalcin-like protein 1
UniProt Gene Name
HPCAL1
UniProt Synonym Gene Names
BDR1; VILIP-3
UniProt Entry Name
HPCL1_HUMAN

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Product Notes

The HPCAL1 hpcal1 (Catalog #AAA288188) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-32. The VILIP3 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VILIP3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HPCAL1 hpcal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VILIP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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