<
WB (Western Blot)
(Anti- Vimentin Picoband antibody, AAA46545, Western blottingAll lanes: Anti Vimentin (AAA46545) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 54KDObserved bind size: 54KD)
Vimentin Polyclonal Antibody | anti-VIM antibody
Anti-Vimentin Antibody
Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Vimentin, Antibody; Anti-Vimentin Antibody; Vimentin; CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; vimentin; anti-VIM antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
466
Applicable Applications for anti-VIM antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin (435-466aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti- Vimentin Picoband antibody, AAA46545, Western blottingAll lanes: Anti Vimentin (AAA46545) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 54KDObserved bind size: 54KD)
WB (Western Blot)
(Anti- Vimentin Picoband antibody, AAA46545, Western blottingAll lanes: Anti Vimentin (AAA46545) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Mouse Kidney Tissue Lysate at 50ugPredicted bind size: 54KDObserved bind size: 54KD)
IHC (Immunohistochemistry)
(Anti- Vimentin Picoband antibody, AAA46545, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemistry)
(Anti- Vimentin Picoband antibody, AAA46545, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- Vimentin Picoband antibody, AAA46545, IHC(P)IHC(P): Rat Cardiac Muscle Tissue)
IHC (Immunohistochemisry)
(Anti- Vimentin Picoband antibody, AAA46545, IHC(P)IHC(P): Rat Cardiac Muscle Tissue)
IHC (Immunohiostchemistry)
(Anti- Vimentin Picoband antibody, AAA46545, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue)
IHC (Immunohiostchemistry)
(Anti- Vimentin Picoband antibody, AAA46545, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue)
WB (Western Blot)
(Anti- Vimentin Picoband antibody, AAA46545, Western blottingAll lanes: Anti Vimentin (AAA46545) at 0.5ug/mlLane 1: HT1080 Whole Cell Lysate at 40ugLane 2: NIH Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: HUT Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: Human Placenta Tissue Lysate at 50ugLane 8: Rat Testis Tissue Lysate at 50ugLane 9: Mouse Testis Tissue Lysate at 50ugPredicted bind size: 54KDObserved bind size: 54KD)
WB (Western Blot)
(Anti- Vimentin Picoband antibody, AAA46545, Western blottingAll lanes: Anti Vimentin (AAA46545) at 0.5ug/mlLane 1: HT1080 Whole Cell Lysate at 40ugLane 2: NIH Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: HUT Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: Human Placenta Tissue Lysate at 50ugLane 8: Rat Testis Tissue Lysate at 50ugLane 9: Mouse Testis Tissue Lysate at 50ugPredicted bind size: 54KDObserved bind size: 54KD)
Related Product Information for anti-VIM antibody
Description: Rabbit IgG polyclonal antibody for Vimentin(VIM) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: VIM(vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Background: VIM(vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
References
1. Besarani D, et al. Role of anti-vimentin antibodies in renal transplantation. Transplantation, 2014 Jul 15. 2. Kinloch AJ, et al. Vimentin is a dominant target of in situ humoral immunity in human lupus tubulointerstitial nephritis. Arthritis Rheumatol, 2014 Dec. 3. Shirahata A, et al. Serum vimentin methylation as a potential marker for colorectal cancer. Anticancer Res, 2014 Aug.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,652 Da
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Official Synonym Symbols
HEL113; CTRCT30
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
Similar Products
Product Notes
The VIM vim (Catalog #AAA46545) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Vimentin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Vimentin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.