WB (Western Blot)
(Detection of human vPARP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-vPARP antibody AAA211696 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human vPARP Polyclonal Antibody | anti-vPARP antibody
Rabbit anti-vPARP Antibody, Affinity Purified
Gene Names
PARP4; PH5P; p193; ARTD4; PARPL; VPARP; VWA5C; PARP-4; VAULT3; ADPRTL1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
vPARP, Antibody; Rabbit anti-vPARP Antibody, Affinity Purified; VPARP; PH5P; poly [ADP-ribose] polymerase 4; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase-like 1; vault poly(ADP-ribose) polymerase; vault protein, 193-kDa; von Willebrand factor A domain containing 5C; PARP-4; VWA5C; VAULT3; ADP-ribosyltransferase diphtheria toxin-like 4; PARP-related; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1; PARP-related/IalphaI-related H5/proline-rich; ADPRTL1; ARTD4; H5 proline-rich; I-alpha-I-related; p193; PARPL; 193 kDa vault protein; Poly [ADP-ribose] polymerase 4; poly(ADP-ribose) polymerase family member 4; anti-vPARP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-vPARP antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1674 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human vPARP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-vPARP antibody AAA211696 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human vPARP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-vPARP antibody AAA211696 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human vPARP by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-vPARP (Cat. No. AAA211696 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human vPARP by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-vPARP (Cat. No. AAA211696 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-vPARP antibody
PARP4 [poly(ADP-ribose) polymerase family, member 4] catalyzes the formation of ADP-ribose polymers covalently attached to proteins using NAD+ as a substrate. There are 6 mammalian PARP enzymes: PARP1, PARP2, PARP3, PARP4/vPARP, PARP5/Tankyrase-1, and PARP6/Tankyrase-2. PARP4 is also known as vault PARP (vPARP) due to its association with the vault particle, a 13MDa cytoplasmic ribonucleoprotein complex whose barrel-shaped structure resembles arched cathedral ceilings. vPARP has also been identified separate from the vault particle. It has been localized to the nuclear pore, mitotic spindle, and cytoplasm. It also has been shown to associate with telomerase activity. Studies of vPARP knockout mice show an increased susceptibility to carcinogen-induced tumorigenesis.
Product Categories/Family for anti-vPARP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
192,595 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly (ADP-ribose) polymerase family, member 4
NCBI Official Symbol
PARP4
NCBI Official Synonym Symbols
PH5P; p193; ARTD4; PARPL; VPARP; VWA5C; PARP-4; VAULT3; ADPRTL1
NCBI Protein Information
poly [ADP-ribose] polymerase 4; poly [ADP-ribose] polymerase 4; PARP-related; H5 proline-rich; I-alpha-I-related; 193 kDa vault protein; vault protein, 193-kDa; poly(ADP-ribose) synthetase; vault poly(ADP-ribose) polymerase; poly(ADP-ribosyl)transferase-l
UniProt Protein Name
Poly [ADP-ribose] polymerase 4
UniProt Gene Name
PARP4
UniProt Synonym Gene Names
ADPRTL1; KIAA0177; PARPL; PARP-4; ARTD4; PH5P; VPARP
UniProt Entry Name
PARP4_HUMAN
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Product Notes
The vPARP parp4 (Catalog #AAA211696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-vPARP Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's vPARP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the vPARP parp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "vPARP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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