IP (Immunoprecipitation)
(VPS25 was immunoprecipitated using:; Lane A:0.5 mg THP-1 Whole Cell Lysate; 4 uL anti-VPS25 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-VPS25 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 21 kDa; Observed band size :21 kDa)
Rabbit anti-Human VPS25 Polyclonal Antibody | anti-VPS25 antibody
VPS25 Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry
Synonyms
VPS25, Antibody; VPS25 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-DERP9 Antibody; Anti-EAP20 Antibody; Anti-FAP20 Antibody; anti-VPS25 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human VPS25
Applicable Applications for anti-VPS25 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry)
Preparation
Produced in rabbits immunized with E Coli-derived Human VPS25 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(VPS25 was immunoprecipitated using:; Lane A:0.5 mg THP-1 Whole Cell Lysate; 4 uL anti-VPS25 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-VPS25 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 21 kDa; Observed band size :21 kDa)
IP (Immunoprecipitation)
(VPS25 was immunoprecipitated using:; Lane A:0.5 mg THP-1 Whole Cell Lysate; 4 uL anti-VPS25 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-VPS25 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 21 kDa; Observed band size :21 kDa)
IHC (Immunohistochemistry)
(Immunochemical staining of human VPS25 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human VPS25 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-VPS25 antibody
Vacuolar protein sorting 25 (Vps25), a component of ESCRT-II (Endosomal Sorting Complex Required for Transport II), directs preferential endosome-mediated modulation of FGF signaling in limbs. Vps25p is the product of yeast gene VPS25 and is found in an endosomal sorting complex required for transport (ESCRT)-II, along with Vps22p and Vps36p. This complex is essential for sorting ubiquitinated biosynthetic and endosomal cargoes into endosomes. In Drosophila, mutations of the ESCRT-II component vps25 cause endosomal defects leading to accumulation of Notch protein and increased Notch pathway activity. In Drosophila imaginal epithelia, cells mutant for the endocytic neoplastic tumor suppressor gene vps25 stimulate nearby untransformed cells to express Drosophila Inhibitor-of-Apoptosis-Protein-1 (DIAP-1), conferring resistance to apoptosis non-cell autonomously.
Product Categories/Family for anti-VPS25 antibody
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Product Notes
The VPS25 (Catalog #AAA258649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS25 Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VPS25 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the VPS25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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