WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using VPS33B antibody.)
Rabbit VPS33B Polyclonal Antibody | anti-VPS33B antibody
VPS33B Antibody
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
VPS33B, Antibody; VPS33B Antibody; FLJ14848; hVPS 33B; hVPS33B; Vacuolar protein sorting 33 homolog B (yeast); Vacuolar protein sorting 33 homolog B; Vacuolar protein sorting 33B (yeast); Vacuolar protein sorting 33B; Vacuolar protein sorting associated protein 33B; VPS 33B; anti-VPS33B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Polyclonal
Isotype
IgG
Specificity
VPS33B antibody detects endogenous levels of VPS33B.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
590
Applicable Applications for anti-VPS33B antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human VPS33B.
Subcellular Location
Late Endosome Membrane; Peripheral Membrane Protein; Cytoplasmic Side. Lysosome Membrane; Peripheral Membrane Protein; Cytoplasmic Side. Note: Cytoplasmic, Peripheral Membrane Protein associated with late endosomes/lysosomes. Colocalizes with M.tuberculosis PtpA in the cytosol of tuberculosis-infected macrophages and associates with phagosomes. Colocalizes in clusters with VIPAS39 at cytoplasmic organelles.
Tissue Specificity
Ubiquitous; highly expressed in testis and low expression in the lung.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Dog (100%), Chicken (83%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using VPS33B antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells, using VPS33B antibody.)
IHC (Immunohistochemistry)
(AAA323005 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA323005 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-VPS33B antibody
Function: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages (PubMed:18474358). Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (By similarity).
Subunit Structure: Interacts with RAB11A and VIPAS39. Associates with adaptor protein complex 3 (AP-3), clathrin:AP-3 and clathrin:HGS complexes (PubMed:21411634). Interacts with M.tuberculosis PtpA.
Post-translational Modifications: Phosphorylated on tyrosine residues. Dephosphorylation by M.tuberculosis PtpA is necessary to induce the reduction of host phagolysosome fusion in M.tuberculosis-infected macrophages.
Similarity: Belongs to the STXBP/unc-18/SEC1 family.
Subunit Structure: Interacts with RAB11A and VIPAS39. Associates with adaptor protein complex 3 (AP-3), clathrin:AP-3 and clathrin:HGS complexes (PubMed:21411634). Interacts with M.tuberculosis PtpA.
Post-translational Modifications: Phosphorylated on tyrosine residues. Dephosphorylation by M.tuberculosis PtpA is necessary to induce the reduction of host phagolysosome fusion in M.tuberculosis-infected macrophages.
Similarity: Belongs to the STXBP/unc-18/SEC1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 71 kDa
Predicted: 71 kDa
Predicted: 71 kDa
NCBI Official Full Name
vacuolar protein sorting-associated protein 33B isoform 2
NCBI Official Synonym Full Names
VPS33B, late endosome and lysosome associated
NCBI Official Symbol
VPS33B
NCBI Protein Information
vacuolar protein sorting-associated protein 33B
UniProt Protein Name
Vacuolar protein sorting-associated protein 33B
UniProt Gene Name
VPS33B
UniProt Synonym Gene Names
hVPS33B
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Product Notes
The VPS33B vps33b (Catalog #AAA323005) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS33B Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's VPS33B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the VPS33B vps33b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS33B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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