Rabbit VPS36 Polyclonal Antibody | anti-VPS36 antibody

VPS36 Antibody

Cat. Num. AAA329164

• Gene Names: VPS36; EAP45; C13orf9; CGI-145
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: VPS36, Antibody; VPS36 Antibody; C13orf9; CGI 145; EAP45; ELL associated protein of 45 kDa; ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36; Vacuolar protein sorting associated protein 36; Vacuolar protein-sorting-associated protein 36; Vps36; VPS36_HUMAN; anti-VPS36 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VPS36 Antibody detects endogenous levels of total VPS36.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 386

• Applicable Applications for anti-VPS36 antibody: ELISA
• Immunogen: A synthesized peptide derived from human VPS36, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(AAA329164 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from Mouse spleen, using VPS36 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-VPS36 antibody

Function: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.

Subcellular Location: Cytoplasm. Endosome. Late endosome. Membrane. Nucleus. Note: Colocalizes with ubiquitinated proteins on late endosomes. Recruited to the endosome membrane to participate in vesicle formation.

Subunit Structure: Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25 (PubMed:14519844). Interacts with VPS25, SNF8, TSG101 and CHMP6 (PubMed:14505570, PubMed:14519844, PubMed:16973552, PubMed:18539118). Interacts (via GLUE domain) with ubiquitin (PubMed:17057716). Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes (PubMed:17010938). Interacts with ECPAS (PubMed:20682791).

Similarity: The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.Belongs to the VPS36 family.

NCBI and Uniprot Product Information

• NCBI GI #: 71051598
• NCBI GeneID: 51028
• NCBI Accession #: NP_057159
• NCBI GenBank Nucleotide #: NP_057159.2
• UniProt Accession #: Q86VN1
• Molecular Weight: Observed Molecular Weight: (Observed)43kD.; Predicted Molecular Weight: (Calculated)44kDa.
• NCBI Official Full Name: vacuolar protein-sorting-associated protein 36 isoform 1
• NCBI Official Synonym Full Names: vacuolar protein sorting 36 homolog
• NCBI Official Symbol: VPS36
• NCBI Official Synonym Symbols: EAP45; C13orf9; CGI-145
• NCBI Protein Information: vacuolar protein-sorting-associated protein 36
• UniProt Protein Name: Vacuolar protein-sorting-associated protein 36
• UniProt Gene Name: VPS36
• UniProt Synonym Gene Names: C13orf9; EAP45
• UniProt Entry Name: VPS36_HUMAN