Rabbit VPS4A Polyclonal Antibody | anti-VPS4A antibody

VPS4A Antibody

Cat. Num. AAA231606

• Gene Names: VPS4A; SKD1; SKD2; VPS4; SKD1A; VPS4-1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Antigen Affinity Purified
• Synonyms: VPS4A, Antibody; VPS4A Antibody; Vacuolar protein sorting-associated protein 4A; Protein SKD2; VPS4-1; hVPS4; VPS4A; anti-VPS4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 437

• Applicable Applications for anti-VPS4A antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Species: Human
• Immunogen: Recombinant human Vacuolar protein sorting-associated protein 4A protein (N-120AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-21461 / sc-21463 / sc-32922
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded human testis tissue using AAA231606 at dilution 1:100)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human lung tissue using AAA231606 at dilution 1:100)

WB (Western Blot)

(Western blotAll lanes:  VPS4Aantibody at 4.28ug/mlLane 1 : Mouse kidney tissueLane 2 : Rat gonadal tissueLane 3 : Mouse brain tissueLane 4 : U87 whole cell lysateLane 5 : MCF7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 49 kDaObserved band size: 49 kDa)

Related Product Information for anti-VPS4A antibody

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

NCBI and Uniprot Product Information

• NCBI GI #: 7019569
• NCBI GeneID: 27183
• NCBI Accession #: NP_037377.1
• NCBI GenBank Nucleotide #: NM_013245.2
• UniProt Accession #: Q9UN37; Q8TF07; Q9UI03; Q9Y582; B2RCB7
• Molecular Weight: 48,898 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 4A
• NCBI Official Synonym Full Names: vacuolar protein sorting 4 homolog A
• NCBI Official Symbol: VPS4A
• NCBI Official Synonym Symbols: SKD1; SKD2; VPS4; SKD1A; VPS4-1
• NCBI Protein Information: vacuolar protein sorting-associated protein 4A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 4A
• UniProt Gene Name: VPS4A
• UniProt Synonym Gene Names: hVPS4
• UniProt Entry Name: VPS4A_HUMAN