IHC (Immunohiostchemistry)
(Immunohistochemical analysis of AAA284379 on paraffin-embedded human testis tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)
Rabbit anti-Mouse VSIG8 Polyclonal Antibody | anti-VSIG8 antibody
VSIG8 Antibody (C-term)
Reactivity
Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
VSIG8, Antibody; VSIG8 Antibody (C-term); V-set and immunoglobulin domain-containing protein 8; VSIG8; C1orf204; anti-VSIG8 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This VSIG8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the C-terminal region of human VSIG8.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
331-360
Sequence Length
235
Applicable Applications for anti-VSIG8 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Precautions
VSIG8 Antibody (C-term) is for research use only
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of AAA284379 on paraffin-embedded human testis tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of AAA284379 on paraffin-embedded human testis tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)
WB (Western Blot)
(All lanes : Anti-VSIG8 Antibody (C-term) at 1:2000 dilution Lane 1: RD whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-VSIG8 Antibody (C-term) at 1:2000 dilution Lane 1: RD whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-VSIG8 antibody
VSIG8 contains 2 Ig-like V-type (immunoglobulin-like) domains. VSIG8 is single-pass type I membrane protein. The function of the VSIG8 protein remains unknown.
Product Categories/Family for anti-VSIG8 antibody
References
Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
V-set and immunoglobulin domain-containing protein 8
NCBI Official Synonym Full Names
V-set and immunoglobulin domain containing 8
NCBI Official Symbol
VSIG8
NCBI Protein Information
V-set and immunoglobulin domain-containing protein 8
UniProt Protein Name
V-set and immunoglobulin domain-containing protein 8
UniProt Gene Name
VSIG8
UniProt Synonym Gene Names
C1orf204
UniProt Entry Name
VSIG8_HUMAN
Similar Products
Product Notes
The VSIG8 vsig8 (Catalog #AAA284379) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 331-360. The VSIG8 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VSIG8 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the VSIG8 vsig8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VSIG8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.