Rabbit anti-Human WAS Polyclonal Antibody | anti-WAS antibody

WAS Polyclonal Antibody

Cat. Num. AAA37103

• Gene Names: WAS; THC; IMD2; SCNX; THC1; WASP
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purification
• Synonyms: WAS, Antibody; WAS Polyclonal Antibody; WAS; IMD2; THC; THC1; WASP; anti-WAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 502

• Applicable Applications for anti-WAS antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human WAS
• Calculated Molecular Weight: 53kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells using WAS antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using WAS Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)

Related Product Information for anti-WAS antibody

Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).

Product Categories/Family for anti-WAS antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 1722836
• NCBI GeneID: 7454
• UniProt Accession #: P42768; Q9BU11; Q9UNJ9
• Molecular Weight: 502
• NCBI Official Full Name: Wiskott-Aldrich syndrome protein
• NCBI Official Synonym Full Names: Wiskott-Aldrich syndrome
• NCBI Official Symbol: WAS
• NCBI Official Synonym Symbols: THC; IMD2; SCNX; THC1; WASP
• NCBI Protein Information: wiskott-Aldrich syndrome protein; eczema-thrombocytopenia; thrombocytopenia 1 (X-linked)
• UniProt Protein Name: Wiskott-Aldrich syndrome protein
• UniProt Gene Name: WAS
• UniProt Synonym Gene Names: IMD2; WASp
• UniProt Entry Name: WASP_HUMAN