WB (Western Blot)
(Western blot analysis of Phospho-WASP (Tyr291) in lysates of HepG2, using Phospho-WASP (Tyr291) Antibody.)
Rabbit WASP Polyclonal Antibody | anti-WASP antibody
Phospho-WASP (Tyr291) Antibody
Gene Names
WAS; THC; IMD2; SCNX; THC1; WASP; WASPA
Reactivity
Human, MousePredicted: Sheep, Rabbit, Dog, Xenopus
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
WASP, Antibody; Phospho-WASP (Tyr291) Antibody; Eczema thrombocytopenia; IMD2; SCNX; THC; THC1; Thrombocytopenia 1 (X linked); U42471; Was; WASp; WASP_HUMAN; Wiskott Aldrich syndrome (eczema thrombocytopenia); Wiskott Aldrich syndrome; Wiskott Aldrich syndrome protein; Wiskott-Aldrich syndrome protein; anti-WASP antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Sheep, Rabbit, Dog, Xenopus
Predicted: Sheep, Rabbit, Dog, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-WASP (Tyr291) Antibody detects endogenous levels of WASP only when phosphorylated at Tyr291.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
502
Applicable Applications for anti-WASP antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human WASP around the phosphorylation site of Tyr291.
Tissue Specificity
Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
Subcellular Location
Cytoskeleton
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-WASP (Tyr291) in lysates of HepG2, using Phospho-WASP (Tyr291) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-WASP (Tyr291) in lysates of HepG2, using Phospho-WASP (Tyr291) Antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-WASP antibody
Description: Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria.
Post Translational Modifications: Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation.
Subunit Structure: Interacts with NCK1 (via SH3 domains) (By similarity). Interacts with CDC42, RAC, NCK, HCK, FYN, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Binds the Arp2/3 complex. Interacts (via C-terminus) with ALDOA. Interacts with E.coli effector protein EspF(U).
Similarity: The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand. The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
Post Translational Modifications: Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation.
Subunit Structure: Interacts with NCK1 (via SH3 domains) (By similarity). Interacts with CDC42, RAC, NCK, HCK, FYN, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Binds the Arp2/3 complex. Interacts (via C-terminus) with ALDOA. Interacts with E.coli effector protein EspF(U).
Similarity: The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand. The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 53kd
Predicted: 53 kDa
Predicted: 53 kDa
NCBI Official Full Name
wiskott-Aldrich syndrome protein
NCBI Official Synonym Full Names
WASP actin nucleation promoting factor
NCBI Official Symbol
WAS
NCBI Official Synonym Symbols
THC; IMD2; SCNX; THC1; WASP; WASPA
NCBI Protein Information
wiskott-Aldrich syndrome protein
UniProt Protein Name
Wiskott-Aldrich syndrome protein
UniProt Gene Name
WAS
UniProt Synonym Gene Names
IMD2; WASp
UniProt Entry Name
WASP_HUMAN
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Product Notes
The WASP was (Catalog #AAA328452) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-WASP (Tyr291) Antibody reacts with Human, Mouse Predicted: Sheep, Rabbit, Dog, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's WASP can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the WASP was for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WASP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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