<
ICC (Immunocytochemistry)
(Anti-WISP1 antibody, AAA45497, ICCICC: MCF-7 Cell)
Rabbit WISP1 Polyclonal Antibody | anti-WISP1 antibody
Anti-WISP1 antibody
Gene Names
WISP1; CCN4; WISP1c; WISP1i; WISP1tc
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
WISP1, Antibody; Anti-WISP1 antibody; WNT1-inducible-signaling pathway protein 1; WNT1 inducible signaling pathway protein 1; CCN 4 antibody; CCN family member 4 antibody; CCN4 antibody; WISP 1 antibody; WISP 1c antibody; WISP 1i antibody; WISP 1tc antibody; WISP1c antibody; WISP1i antibody; WISP1tc antibody; WNT 1 induced secreted protein 1 antibody; Wnt 1 induced secreted protein antibody; WNT 1 inducible signaling pathway protein 1 antibody; Wnt 1 signaling pathway protein 1 antibody; Wnt-1 inducible signaling pathway protein 1 antibody; WNT1 induced secreted protein 1 antibody; Wnt1 induced secreted protein antibody; WNT1 inducible signaling pathway protein 1 antibody; Wnt1 signaling pathway protein 1 antibody; WNT1-inducible-signaling pathway protein 1 antibody; anti-WISP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
367
Applicable Applications for anti-WISP1 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human WISP1 (354-367aa ADLESYPDFSEIAN), different from the related mouse and rat sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(Anti-WISP1 antibody, AAA45497, ICCICC: MCF-7 Cell)
ICC (Immunocytochemistry)
(Anti-WISP1 antibody, AAA45497, ICCICC: MCF-7 Cell)
WB (Western Blot)
(Anti-WISP1 antibody, AAA45497, Western blottingAll lanes: Anti WISP1 (AAA45497) at 0.5ug/mlLane 1: Rat Heart Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Lung Tissue Lysate at 50ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: A431 Whole Cell Lysate at 40ugLane 6: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 40KDObserved bind size: 40KD)
WB (Western Blot)
(Anti-WISP1 antibody, AAA45497, Western blottingAll lanes: Anti WISP1 (AAA45497) at 0.5ug/mlLane 1: Rat Heart Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Rat Lung Tissue Lysate at 50ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: A431 Whole Cell Lysate at 40ugLane 6: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 40KDObserved bind size: 40KD)
IHC (Immunohistochemisry)
(Anti-WISP1 antibody, AAA45497, IHC(P)IHC(P): Human Placenta Tissue)
IHC (Immunohistochemisry)
(Anti-WISP1 antibody, AAA45497, IHC(P)IHC(P): Human Placenta Tissue)
IHC (Immunohiostchemistry)
(Anti-WISP1 antibody, AAA45497, IHC(P)IHC(P): Rat Pancreas Tissue)
IHC (Immunohiostchemistry)
(Anti-WISP1 antibody, AAA45497, IHC(P)IHC(P): Rat Pancreas Tissue)
IHC (Immunohistochemistry)
(Anti-WISP1 antibody, AAA45497, IHC(P)IHC(P): Rat Kidney Tissue)
IHC (Immunohistochemistry)
(Anti-WISP1 antibody, AAA45497, IHC(P)IHC(P): Rat Kidney Tissue)
Related Product Information for anti-WISP1 antibody
Description: Rabbit IgG polyclonal antibody for WNT1-inducible-signaling pathway protein 1(WISP1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed(Tanaka et al., 2001). By use of radiation hybrid mapping panels, Pennica et al.(1998) mapped the WISP1 to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. Pennica et al.(1998) found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.
Background: WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed(Tanaka et al., 2001). By use of radiation hybrid mapping panels, Pennica et al.(1998) mapped the WISP1 to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. Pennica et al.(1998) found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,550 Da
NCBI Official Full Name
WNT1-inducible-signaling pathway protein 1
NCBI Official Synonym Full Names
WNT1 inducible signaling pathway protein 1
NCBI Official Symbol
WISP1
NCBI Official Synonym Symbols
CCN4; WISP1c; WISP1i; WISP1tc
NCBI Protein Information
WNT1-inducible-signaling pathway protein 1; CTC-458A3.8; CCN family member 4; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1
UniProt Protein Name
WNT1-inducible-signaling pathway protein 1
UniProt Gene Name
WISP1
UniProt Synonym Gene Names
CCN4; WISP-1
UniProt Entry Name
WISP1_HUMAN
Similar Products
Product Notes
The WISP1 wisp1 (Catalog #AAA45497) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-WISP1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WISP1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the WISP1 wisp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WISP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.