IHC (Immunohistochemisry)
(WNT16 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WNT16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit WNT16 Polyclonal Antibody | anti-WNT16 antibody
WNT16 Antibody (C-term)
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
WNT16, Antibody; WNT16 Antibody (C-term); Protein Wnt-16; WNT16; anti-WNT16 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This WNT16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-265 amino acids from the C-terminal region of human WNT16.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
236-265
Sequence Length
355
Applicable Applications for anti-WNT16 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(WNT16 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WNT16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(WNT16 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WNT16 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(WNT16 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the WNT16 antibody detected the WNT16 protein (arrow).)
WB (Western Blot)
(WNT16 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the WNT16 antibody detected the WNT16 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody (C-term). AAA284580 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody (C-term). AAA284580 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-WNT16 antibody
The WNT gene family consists of structurally related genes
which encode secreted signaling proteins. These proteins have been
implicated in oncogenesis and in several developmental processes,
including regulation of cell fate and patterning during
embryogenesis. This gene is a member of the WNT gene family. It
contains two transcript variants diverging at the 5' termini. These
two variants are proposed to be the products of separate promoters
and not to be splice variants from a single promoter. They are
differentially expressed in normal tissues, one of which (variant
2) is expressed at significant levels only in the pancreas, whereas
another one (variant 1) is expressed more ubiquitously with highest
levels in adult kidney, placenta, brain, heart, and spleen.
which encode secreted signaling proteins. These proteins have been
implicated in oncogenesis and in several developmental processes,
including regulation of cell fate and patterning during
embryogenesis. This gene is a member of the WNT gene family. It
contains two transcript variants diverging at the 5' termini. These
two variants are proposed to be the products of separate promoters
and not to be splice variants from a single promoter. They are
differentially expressed in normal tissues, one of which (variant
2) is expressed at significant levels only in the pancreas, whereas
another one (variant 1) is expressed more ubiquitously with highest
levels in adult kidney, placenta, brain, heart, and spleen.
Product Categories/Family for anti-WNT16 antibody
References
Binet, R., et al. Cancer Res. 69(24):9183-9191(2009)
Memarian, A., et al. Leuk. Lymphoma 50(12):2061-2070(2009)
Nygren, M.K., et al. Exp. Hematol. 37(2):225-233(2009)
Teh, M.T., et al. J. Cell. Sci. 120 (PT 2), 330-339 (2007) :
Casagrande, G., et al. Haematologica 91(6):765-771(2006)
Memarian, A., et al. Leuk. Lymphoma 50(12):2061-2070(2009)
Nygren, M.K., et al. Exp. Hematol. 37(2):225-233(2009)
Teh, M.T., et al. J. Cell. Sci. 120 (PT 2), 330-339 (2007) :
Casagrande, G., et al. Haematologica 91(6):765-771(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40690
NCBI Official Full Name
protein Wnt-16 isoform 2
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 16
NCBI Official Symbol
WNT16
NCBI Protein Information
protein Wnt-16
UniProt Protein Name
Protein Wnt-16
UniProt Gene Name
WNT16
UniProt Entry Name
WNT16_HUMAN
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Product Notes
The WNT16 wnt16 (Catalog #AAA284580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 236-265. The WNT16 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's WNT16 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the WNT16 wnt16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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