FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using WNT5B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit WNT5B Polyclonal Antibody | anti-WNT5B antibody
WNT5B Antibody (Center)
Reactivity
Human, mouse (Predicted Reactivity: Rabbit, Rat, Xenopus)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
WNT5B, Antibody; WNT5B Antibody (Center); Protein Wnt-5b; WNT5B; anti-WNT5B antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rabbit, Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This WNT5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the Central region of human WNT5B.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
153-182
Sequence Length
359
Applicable Applications for anti-WNT5B antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Cellular Location
Secreted, extracellular space, extracellular matrix
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using WNT5B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using WNT5B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle with WNT5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle with WNT5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of WNT5B Antibody (Center) in mouse bladder tissue lysates (35ug/lane). WNT5B (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of WNT5B Antibody (Center) in mouse bladder tissue lysates (35ug/lane). WNT5B (arrow) was detected using the purified Pab.)
Related Product Information for anti-WNT5B antibody
WNT5B is a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively.
Product Categories/Family for anti-WNT5B antibody
References
Saitoh,T. et.al., Int. J. Mol. Med. 10 (3), 345-349 (2002) Yates,K.E. et.al., Osteoarthr. Cartil. 12 (6), 497-505 (2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40323
NCBI Official Full Name
protein Wnt-5b
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 5B
NCBI Official Symbol
WNT5B
NCBI Protein Information
protein Wnt-5b
UniProt Protein Name
Protein Wnt-5b
UniProt Gene Name
WNT5B
UniProt Entry Name
WNT5B_HUMAN
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Product Notes
The WNT5B wnt5b (Catalog #AAA288810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 153-182. The WNT5B Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Rabbit, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's WNT5B can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the WNT5B wnt5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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