IF (Immunofluorescence)
(AAA327775 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA327775 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue))
Rabbit anti-Human, Mouse WNT9A Polyclonal Antibody | anti-WNT9A antibody
WNT9A Antibody
Gene Names
WNT9A; WNT14
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
Purity
Peptide affinity purification
Synonyms
WNT9A, Antibody; WNT9A Antibody; MGC138165; MGC141991; Protein Wnt-14; Protein Wnt-9a; RP23-378H5.1; Wingless related MMTV integration site 9A; Wingless type MMTV integration site 9A; Wingless type MMTV integration site family, member 9A; wingless-type MMTV integration site family, member 14; Wnt 14; Wnt 9a; WNT14; WNT9A; WNT9A_HUMAN; anti-WNT9A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
WNT9A antibody detects endogenous levels of total WNT9A
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Sequence Length
365
Applicable Applications for anti-WNT9A antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human WNT9A, corresponding to a region within C-terminal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA327775 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA327775 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue))
IF (Immunofluorescence)
(AAA327775 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA327775 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue))
WB (Western Blot)
(Western blot analysis of WNT9A using K562 whole cell lysates)
WB (Western Blot)
(Western blot analysis of WNT9A using K562 whole cell lysates)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40 kDa
NCBI Official Full Name
protein Wnt-9a
NCBI Official Synonym Full Names
Wnt family member 9A
NCBI Official Symbol
WNT9A
NCBI Official Synonym Symbols
WNT14
NCBI Protein Information
protein Wnt-9a
UniProt Protein Name
Protein Wnt-9a
UniProt Gene Name
WNT9A
UniProt Synonym Gene Names
WNT14
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Product Notes
The WNT9A wnt9a (Catalog #AAA327775) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WNT9A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's WNT9A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the WNT9A wnt9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT9A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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