WB (Western Blot)
(Detection of human XPF/ERCC4 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, LNCaP, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPF/ERCC4 antibody (AAA211413 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human XPF/ERCC4 Polyclonal Antibody | anti-XPF/ERCC4 antibody
Rabbit anti-XPF/ERCC4 Antibody, Affinity Purified
Gene Names
ERCC4; XPF; RAD1; FANCQ; ERCC11
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
XPF/ERCC4, Antibody; Rabbit anti-XPF/ERCC4 Antibody, Affinity Purified; ERCC11; FANCQ; XFEPS; xeroderma pigmentosum, complementation group F; xeroderma pigmentosum group F-complementing protein; RAD1; XPF; excision-repair, complementing defective, in Chinese hamster; excision repair cross-complementation group 4; DNA repair protein complementing XP-F cells; DNA repair endonuclease XPF; DNA excision repair protein ERCC-4; excision repair cross-complementing rodent repair deficiency, complementation group 4; ERCC excision repair 4, endonuclease catalytic subunit; anti-XPF/ERCC4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-XPF/ERCC4 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 866 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human XPF/ERCC4 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, LNCaP, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPF/ERCC4 antibody (AAA211413 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human XPF/ERCC4 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, LNCaP, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPF/ERCC4 antibody (AAA211413 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human XPF/ERCC4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XPF/ERCC4 antibody (AAA211413 lot 2) used for IP at 6 ug per reaction. XPF/ERCC4 was also immunoprecipitated by a previous lot of this antibody (AAA211413 lot 1) and a second antibody against a different epitope of XPF/ERCC4 (BL5427). For blotting immunoprecipitated XPF/ERCC4, AAA211413 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human XPF/ERCC4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XPF/ERCC4 antibody (AAA211413 lot 2) used for IP at 6 ug per reaction. XPF/ERCC4 was also immunoprecipitated by a previous lot of this antibody (AAA211413 lot 1) and a second antibody against a different epitope of XPF/ERCC4 (BL5427). For blotting immunoprecipitated XPF/ERCC4, AAA211413 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-XPF/ERCC4 antibody
XPF/ERCC4 is suggested to play a role in the repair of DNA double-strand breaks (DSB), homologous recombination, and gene conversion via single-strand annealing (SSA). XPF/ERCC4 is an endonuclease that incises 5-prime DNA. Defects in XPF/ERCC4 cause xeroderma pigmentosum VI (XP6) an autosomal recessive disease characterized by hypersensitivity to sunlight and a predisposition to skin cancer as well as neurological abnormalities. Defects in XPF/ERCC4 are also responsible for XFE progeroid syndrome, a syndrome characterized by dwarfism, cachexia, and microcephaly.
Product Categories/Family for anti-XPF/ERCC4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
104,486 Da
NCBI Official Full Name
DNA repair endonuclease XPF
NCBI Official Synonym Full Names
excision repair cross-complementing rodent repair deficiency, complementation group 4
NCBI Official Symbol
ERCC4
NCBI Official Synonym Symbols
XPF; RAD1; FANCQ; ERCC11
NCBI Protein Information
DNA repair endonuclease XPF; DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; xeroderma pigmentosum, complementation group F; xeroderma pigmentosum group F-complementing protein; excision-repair, complementing defective, in
UniProt Protein Name
DNA repair endonuclease XPF
UniProt Gene Name
ERCC4
UniProt Synonym Gene Names
ERCC11; XPF
UniProt Entry Name
XPF_HUMAN
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Product Notes
The XPF/ERCC4 ercc4 (Catalog #AAA211413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-XPF/ERCC4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's XPF/ERCC4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the XPF/ERCC4 ercc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XPF/ERCC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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