Rabbit XPO4 Polyclonal Antibody | anti-XPO4 antibody

XPO4 Antibody

Cat. Num. AAA327418

• Gene Names: XPO4; exp4
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: XPO4, Antibody; XPO4 Antibody; Exp4; Exportin-4; exportin4; FLJ13046; KIAA1721; xpo4; XPO4_HUMAN; anti-XPO4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: XPO4 antibody detects endogenous levels of total XPO4
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1151

• Applicable Applications for anti-XPO4 antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human XPO4
• Subcellular Location: Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemisry)

(AAA327418 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence)

(AAA327418 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of XPO4 using HuvEc whole cell lysates)

Related Product Information for anti-XPO4 antibody

Function: Mediates the nuclear export of proteins (cargos) with broad substrate specificity. In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. XPO4 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus.
Subunit Structure: Found in a complex with XPO4, Ran and EIF5A. Found in a complex with XPO4, Ran and SMAD3. Interacts with SMAD3. Interacts with Ran and cargo proteins in a GTP-dependent manner.
Similarity: Belongs to the exportin family.

NCBI and Uniprot Product Information

• NCBI GI #: 148886661
• NCBI GeneID: 64328
• NCBI Accession #: NP_071904.4
• NCBI GenBank Nucleotide #: NM_022459.4
• UniProt Accession #: Q9C0E2; Q5VUZ5; Q8N3V6; Q9H934
• Molecular Weight: Observed: 127 kDa; Predicted: 131 kDa
• NCBI Official Full Name: exportin-4
• NCBI Official Synonym Full Names: exportin 4
• NCBI Official Symbol: XPO4
• NCBI Official Synonym Symbols: exp4
• NCBI Protein Information: exportin-4
• UniProt Protein Name: Exportin-4
• UniProt Gene Name: XPO4
• UniProt Synonym Gene Names: KIAA1721; Exp4