WB (Western Blot)
(Detection of human XRN1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody (AAA210952 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse XRN1 Polyclonal Antibody | anti-XRN1 antibody
Rabbit anti-XRN1 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
XRN1, Antibody; Rabbit anti-XRN1 Antibody, Affinity Purified; 5'-3' exoribonuclease 1; SEP1; strand-exchange protein 1 homolog; anti-XRN1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-XRN1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1656 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human XRN1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody (AAA210952 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human XRN1 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody (AAA210952 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse XRN1 by western blot. Samples: Whole cell lysate (10 ug) from CH27 and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody (AAA210952 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse XRN1 by western blot. Samples: Whole cell lysate (10 ug) from CH27 and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody (AAA210952 lot 5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human XRN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN1 antibody (AAA210952 lot 5) used for IP at 6 ug per reaction. XRN1 was also immunoprecipitated by a previous lot of this antibody (AAA210952 lot 4) and a second antibody against a different epitope of XRN1 (Antibody 2). For blotting immunoprecipitated XRN1, AAA210952 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human XRN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN1 antibody (AAA210952 lot 5) used for IP at 6 ug per reaction. XRN1 was also immunoprecipitated by a previous lot of this antibody (AAA210952 lot 4) and a second antibody against a different epitope of XRN1 (Antibody 2). For blotting immunoprecipitated XRN1, AAA210952 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-XRN1 antibody
5'-3' exoribonuclease 1 (XRN1) is a ribonuclease localized in the cytoplasm that functions in mRNA metabolism and is found in messenger ribonucleoprotein (mRNP) complexes. The mouse and yeast homologs of XRN1 are Dhm2 and SEP1 respectively. In a glial cell line, XRN1 is induced by glial cell line-derived growth factor (GDNF) and associates with microtubules. XRN1 has also been identified as a candidate tumor suppressor gene in osteogenic sarcoma.
Product Categories/Family for anti-XRN1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The XRN1 xrn1 (Catalog #AAA210952) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-XRN1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's XRN1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the XRN1 xrn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XRN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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