FCM/FACS (Flow Cytometry)
(XYLT1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit XYLT1 Polyclonal Antibody | anti-XYLT1 antibody
XYLT1 Antibody (N-term)
Gene Names
XYLT1; XT1; XTI; XT-I; DBQD2; XYLTI; PXYLT1
Reactivity
Human, Mouse, RatPredicted Reactivity: Mouse, Rat
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
XYLT1, Antibody; XYLT1 Antibody (N-term); Xylosyltransferase 1; Peptide O-xylosyltransferase 1; Xylosyltransferase I; XT-I; XylT-I; XYLT1; XT1; anti-XYLT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Mouse, Rat
Predicted Reactivity: Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This XYLT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the N-terminal region of human XYLT1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
126-155
Sequence Length
959
Applicable Applications for anti-XYLT1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(XYLT1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(XYLT1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(All lanes : Anti-XYLT1 Antibody (N-term) at 1:1000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: 293 whole cell lysate Lane 3: U-87MG whole cell lysate Lane 4: PANC-1 whole cell lysate Lane 5: Mouse brain whole cell lysate Lane 6: Rat brain whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-XYLT1 Antibody (N-term) at 1:1000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: 293 whole cell lysate Lane 3: U-87MG whole cell lysate Lane 4: PANC-1 whole cell lysate Lane 5: Mouse brain whole cell lysate Lane 6: Rat brain whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-XYLT1 antibody
This locus encodes a xylosyltransferase enzyme. The
encoded protein catalyzes transfer of UDP-xylose to serine residues
of an acceptor protein substrate. This transfer reaction is
necessary for biosynthesis of glycosaminoglycan chains. Mutations
in this gene have been associated with increased severity of
pseudoxanthoma elasticum.
encoded protein catalyzes transfer of UDP-xylose to serine residues
of an acceptor protein substrate. This transfer reaction is
necessary for biosynthesis of glycosaminoglycan chains. Mutations
in this gene have been associated with increased severity of
pseudoxanthoma elasticum.
Product Categories/Family for anti-XYLT1 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Muller, B., et al. J. Biol. Chem. 284(45):30775-30782(2009)
Ponighaus, C., et al. Am. J. Hypertens. 22(4):432-436(2009)
Ambrosius, M., et al. Clin. Biochem. 42 (1-2), 1-4 (2009) :
Schon, S., et al. J. Med. Genet. 43(9):745-749(2006)
Muller, B., et al. J. Biol. Chem. 284(45):30775-30782(2009)
Ponighaus, C., et al. Am. J. Hypertens. 22(4):432-436(2009)
Ambrosius, M., et al. Clin. Biochem. 42 (1-2), 1-4 (2009) :
Schon, S., et al. J. Med. Genet. 43(9):745-749(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107569
NCBI Official Full Name
xylosyltransferase 1
NCBI Official Synonym Full Names
xylosyltransferase I
NCBI Official Symbol
XYLT1
NCBI Official Synonym Symbols
XT1; XTI; XT-I; DBQD2; XYLTI; PXYLT1
NCBI Protein Information
xylosyltransferase 1
UniProt Protein Name
Xylosyltransferase 1
UniProt Gene Name
XYLT1
UniProt Synonym Gene Names
XT1; XT-I; XylT-I
UniProt Entry Name
XYLT1_HUMAN
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Product Notes
The XYLT1 xylt1 (Catalog #AAA290314) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 126-155. The XYLT1 Antibody (N-term) reacts with Human, Mouse, Rat Predicted Reactivity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's XYLT1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the XYLT1 xylt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XYLT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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