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WB (Western Blot)
(Anti-YFP Ab at 1/2,000 dilution; 293HEK cells transduced with YFP Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
Goat YFP Polyclonal Antibody | anti-YFP antibody
YFP Polyclonal Antibody
Reactivity
Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
This antibody is epitope-affinity purified from goat antiserum.
Synonyms
YFP, Antibody; YFP Polyclonal Antibody; Yellow fluorescent protein antibody; anti-YFP antibody
Host
Goat
Reactivity
Transfected cells proteins
Clonality
Polyclonal
Isotype
IgG
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27kDa by Western Blot. This antibody also recognizes GFP and does not recognize mCherry/red fluorescent protein.
Purity/Purification
This antibody is epitope-affinity purified from goat antiserum.
Form/Format
Polyclonal antibody supplied as a 100 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Sequence Length
239
Applicable Applications for anti-YFP antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, IEM
Application Notes
Western blot: 1:500-1:2,000
Immunofluorescence: 1:50-1:1,000
Immunohistochemistry (paraffin): 1:50-1:1,000
Immunohistochemistry (frozen): 1:50-1:1,000
IEM: 1:50-1:1,000
Immunofluorescence: 1:50-1:1,000
Immunohistochemistry (paraffin): 1:50-1:1,000
Immunohistochemistry (frozen): 1:50-1:1,000
IEM: 1:50-1:1,000
Immunogen
Purified recombinant peptide produced in E. coli
Note
The antibody solution should be gently mixed before use.
Preparation and Storage
For continuous use, store at 2-8 degree C for one-two days. For extended storage, store in -20 degree C freezer. Working dilution samples should be discarded if not used within 12 hours.
WB (Western Blot)
(Anti-YFP Ab at 1/2,000 dilution; 293HEK cells transduced with YFP Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
WB (Western Blot)
(Anti-YFP Ab at 1/2,000 dilution; 293HEK cells transduced with YFP Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
Immunogold
(Immunogold labeling of epithelium cells, in vivo injected with YFP expressing vector;)
Immunogold
(Immunogold labeling of epithelium cells, in vivo injected with YFP expressing vector;)
IF (Immunofluorescence)
(Immunofluorescence – anti-YFP Ab in 293HEK cells transfected with YFP at 1/50 dilution; cells were fixed with 4% of PFA;)
IF (Immunofluorescence)
(Immunofluorescence – anti-YFP Ab in 293HEK cells transfected with YFP at 1/50 dilution; cells were fixed with 4% of PFA;)
Reactivity Chart
Reactivity Chart
Related Product Information for anti-YFP antibody
Goat polyclonal antibody to YFP (yellow fluorescent protein). YFP is a genetic mutant of green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 514/527 nm respectively.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
YFP
Similar Products
Product Notes
The YFP (Catalog #AAA13878) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The YFP Polyclonal Antibody reacts with Transfected cells proteins and may cross-react with other species as described in the data sheet. AAA Biotech's YFP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, IEM. Western blot: 1:500-1:2,000 Immunofluorescence: 1:50-1:1,000 Immunohistochemistry (paraffin): 1:50-1:1,000 Immunohistochemistry (frozen): 1:50-1:1,000 IEM: 1:50-1:1,000. Researchers should empirically determine the suitability of the YFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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