FCM/FACS (Flow Cytometry)
(YWHAZ Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit YWHAZ Polyclonal Antibody | anti-YWHAZ antibody
YWHAZ Antibody (Center)
Gene Names
YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
YWHAZ, Antibody; YWHAZ Antibody (Center); 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAZ; anti-YWHAZ antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-93 amino acids from the Central region of human YWHAZ.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.31 (varies by lot)
Sequence Positions
65-93
Sequence Length
170
Applicable Applications for anti-YWHAZ antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(YWHAZ Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(YWHAZ Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(YWHAZ Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YWHAZ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(YWHAZ Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YWHAZ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of YWHAZ Antibody (Center) in MDA-MB231, A2058 cell line and mouse cerebellum, brain tissue lysates (35ug/lane). YWHAZ (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of YWHAZ Antibody (Center) in MDA-MB231, A2058 cell line and mouse cerebellum, brain tissue lysates (35ug/lane). YWHAZ (arrow) was detected using the purified Pab.)
Related Product Information for anti-YWHAZ antibody
YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
Product Categories/Family for anti-YWHAZ antibody
References
Powell, D.W., et al., Mol. Cell. Biol. 23(15):5376-5387 (2003).
Zhu, P., et al., Biochem. Biophys. Res. Commun. 301(4):991-999 (2003).
Li, Y., et al., J. Biol. Chem. 277(47):44593-44596 (2002).
Wang, H., et al., J. Clin. Endocrinol. Metab. 87(6):2629-2634 (2002).
Nellist, M., et al., J. Biol. Chem. 277(42):39417-39424 (2002).
Zhu, P., et al., Biochem. Biophys. Res. Commun. 301(4):991-999 (2003).
Li, Y., et al., J. Biol. Chem. 277(47):44593-44596 (2002).
Wang, H., et al., J. Clin. Endocrinol. Metab. 87(6):2629-2634 (2002).
Nellist, M., et al., J. Biol. Chem. 277(42):39417-39424 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27745
NCBI Official Full Name
14-3-3 protein zeta/delta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
NCBI Official Symbol
YWHAZ
NCBI Official Synonym Symbols
HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta
NCBI Protein Information
14-3-3 protein zeta/delta
UniProt Protein Name
14-3-3 protein zeta/delta
UniProt Gene Name
YWHAZ
UniProt Synonym Gene Names
KCIP-1
UniProt Entry Name
1433Z_HUMAN
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Product Notes
The YWHAZ ywhaz (Catalog #AAA286808) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 65-93. The YWHAZ Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine, Chicken, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's YWHAZ can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the YWHAZ ywhaz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YWHAZ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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