IP (Immunoprecipitation)
(Detection of human ZAK by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZAK antibody AAA211710 used for IP at 3 ug/mg lysate. ZAK was also immunoprecipitated by rabbit anti-ZAK antibody which recognizes an upstream epitope. For blotting immunoprecipitated ZAK, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human ZAK Polyclonal Antibody | anti-ZAK antibody
Rabbit anti-ZAK Antibody, Affinity Purified
Gene Names
ZAK; pk; AZK; MLT; MRK; MLK7; MLTK; mlklak
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
ZAK, Antibody; Rabbit anti-ZAK Antibody, Affinity Purified; MLT; MLTK; MLTKalpha; MLTKbeta; MRK; Sterile alpha motif- and leucine zipper-containing kinase AZK; ZAK; ZAK1 homolog, leucine zipper and sterile-alpha motif kinase; MLK-related kinase; pk; SFMMP; mitogen-activated protein kinase kinase kinase MLT; MLK-like mitogen-activated protein triple kinase; AZK; CNM6; mitogen-activated protein kinase kinase kinase 20; sterile alpha motif and leucine zipper containing kinase AZK; mixed lineage kinase 7; mixed lineage kinase with a leucine zipper and a sterile alpha motif; Mixed lineage kinase-related kinase; MLK7; mlklak; HCCS-4; Mitogen-activated protein kinase kinase kinase MLT; anti-ZAK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ZAK antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 750 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human ZAK by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZAK antibody AAA211710 used for IP at 3 ug/mg lysate. ZAK was also immunoprecipitated by rabbit anti-ZAK antibody which recognizes an upstream epitope. For blotting immunoprecipitated ZAK, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ZAK by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZAK antibody AAA211710 used for IP at 3 ug/mg lysate. ZAK was also immunoprecipitated by rabbit anti-ZAK antibody which recognizes an upstream epitope. For blotting immunoprecipitated ZAK, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human ZAK by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-ZAK (Cat. No. AAA211710 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ZAK by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-ZAK (Cat. No. AAA211710 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-ZAK antibody
Leucine-zipper and sterile-alpha motif kinase (ZAK) is a mitogen activated protein kinase kinase kinase involved in the S and G2 phase cell cycle checkpoint. ZAK has been shown to activate the stress-activated protein kinase (c-jun N-terminal kinase) pathway and NF-kappaB. It has also been found to be a key mediator of cardiomyocyte hypertrophy and myocardial scars.
Product Categories/Family for anti-ZAK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,155 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase MLT isoform 1
NCBI Official Symbol
ZAK
NCBI Official Synonym Symbols
pk; AZK; MLT; MRK; MLK7; MLTK; mlklak
NCBI Protein Information
mitogen-activated protein kinase kinase kinase MLT; HCCS-4; mixed lineage kinase 7; mixed lineage kinase-related kinase MRK-beta; human cervical cancer suppressor gene 4 protein; MLK-like mitogen-activated protein triple kinase; mixed lineage kinase with
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase MLT
UniProt Gene Name
MLTK
UniProt Synonym Gene Names
ZAK; HCCS-4; MLK-related kinase; MRK
UniProt Entry Name
MLTK_HUMAN
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Product Notes
The ZAK mltk (Catalog #AAA211710) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ZAK Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZAK can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the ZAK mltk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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