IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ZAP 70 (pTyr493) antibody)
Rabbit ZAP 70 Polyclonal Antibody | anti-ZAP70 antibody
Rabbit anti ZAP 70 (pTyr493)
Gene Names
ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Applications
Western Blot, Immunohistochemistry
Purity
Purified whole serum by affinity chromatography.
Synonyms
ZAP 70, Antibody; Rabbit anti ZAP 70 (pTyr493); ZAP 70 (pTyr493) Antibody; anti-ZAP70 antibody
Host
Rabbit
Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified whole serum by affinity chromatography.
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Sequence Length
619
Applicable Applications for anti-ZAP70 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 493 of human ZAP 70
Preperation
Antiserum to ZAP 70 (pTyr493) was raised by repeated immunization of rabbits with highly purified antigen.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ZAP 70 (pTyr493) antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ZAP 70 (pTyr493) antibody)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells untreated or treated with Anisomycin using Rabbit anti ZAP 70 (pTyr493) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells untreated or treated with Anisomycin using Rabbit anti ZAP 70 (pTyr493) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes)
Related Product Information for anti-ZAP70 antibody
Rabbit anti ZAP 70 (pTyr493) antibody recognizes ZAP 70, also known as Zeta-chain-associated protein kinase 70 when phosphorylated at tyrosine 493. ZAP70 is a member of the tyrosine kinase family. ZAP 70 plays a key role in the activation of T cells. ZAP 70 is phosphorylated at tyrosine 493 by Lck in response to T cell receptor stimulation (Wang et al. 2010). Phosphorylation of ZAP 70 at tyrosine 493 fully activates ZAP 70 leading to phosphorylation of downstream targets of the T cell activation pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
55,873 Da
NCBI Official Full Name
Tyrosine-protein kinase ZAP-70
NCBI Official Synonym Full Names
zeta chain of T cell receptor associated protein kinase 70
NCBI Official Symbol
ZAP70
NCBI Official Synonym Symbols
SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
NCBI Protein Information
tyrosine-protein kinase ZAP-70
UniProt Protein Name
Tyrosine-protein kinase ZAP-70
UniProt Gene Name
ZAP70
UniProt Synonym Gene Names
SRK
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Product Notes
The ZAP70 zap70 (Catalog #AAA50309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti ZAP 70 (pTyr493) reacts with Human, Mouse (Expected from Sequence), Rat (Expected from Sequence) and may cross-react with other species as described in the data sheet. AAA Biotech's ZAP 70 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ZAP70 zap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZAP 70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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