IP (Immunoprecipitation)
(ZC3H7A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-ZC3H7A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ZC3H7A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size :110 kDa)
Rabbit anti-Human ZC3H7A Polyclonal Antibody | anti-ZC3H7A antibody
Anti-ZC3H7A Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
ZC3H7A, Antibody; Anti-ZC3H7A Antibody, Rabbit Polyclonal; ZC3H7A Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-HSPC055 Antibody; Anti-ZC3H7 Antibody; Anti-ZC3HDC7 Antibody; anti-ZC3H7A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ZC3H7A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-ZC3H7A antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human ZC3H7A fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ZC3H7A fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(ZC3H7A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-ZC3H7A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ZC3H7A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size :110 kDa)
IP (Immunoprecipitation)
(ZC3H7A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-ZC3H7A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ZC3H7A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size :110 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of ZC3H7A in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZC3H7A polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of ZC3H7A in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZC3H7A polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
WB (Western Blot)
(Anti-ZC3H7A rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:110 kDa)
WB (Western Blot)
(Anti-ZC3H7A rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:110 kDa)
Related Product Information for anti-ZC3H7A antibody
ZC3H7A (Zinc Finger CCCH-Type Containing 7A, also known as ZC3H7; HSPC055; ZC3HDC7), a member of CCCH gene family, is located on human chromosome 16p13.13. ZC3H7A is associated with some diseases, including Endometrial Stromal Tumor and Endometrial Stromal Sarcoma. An important paralog of this gene is ZC3H7B. The CCCH zinc finger motif has been found in proteins from organisms ranging from man to yeast. The CCCH proteins are a large family of zinc finger containing C3H-type motifs and much evidences proved that they may be RNA-binding proteins functioning in RNA processing. In mouse, tristetraprolin, a protein containing two CCCH zinc fingers, binds directly to AU-rich elements within the 3'-untranslated region of target transcripts to facilitate mRNA degradation. Zfp36l2, like its better-known relative TTP, is a mRNA-binding and destabilizing protein, functions in the physiological control of female fertility at the level of early embryonic development. The PIE-1 is an essential regulator of Caenorhabditis elegans germ cell fate that segregates with the germ lineage by inhibition of transcription or activation of protein expression from maternal RNAs.
References
Wang D, et al. (2008) Genome-wide analysis of ccch zinc finger family in arabidopsis and rice. BMC Genomics 9 44.Liang J, et al. (2008) Genome-wide survey and expression profiling of ccch-zinc finger family reveals a functional module in macrophage activation. PLoS One 3 (8): e2880.
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Product Notes
The ZC3H7A (Catalog #AAA256581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZC3H7A Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZC3H7A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ZC3H7A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZC3H7A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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