WB (Western Blot)
(Western blot analysis of extracts of MCF7 cells, using ZCCHC11 antibody.)
Rabbit anti-Human, Mouse ZCCHC11 Polyclonal Antibody | anti-ZCCHC11 antibody
ZCCHC11 Antibody
Gene Names
TUT4; PAPD3; TENT3A; ZCCHC11
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZCCHC11, Antibody; ZCCHC11 Antibody; DKFZp779C1943; EC 2.7.7.52; FLJ42878; KIAA0191; PAP associated domain containing 3; PAPD3; Terminal uridylyltransferase 4; TUT4; TUT4_HUMAN; TUTase 4; ZCCHC 11; Zcchc11; Zinc finger CCHC domain containing 11; Zinc finger CCHC domain containing protein 11; Zinc finger CCHC domain-containing protein 11; anti-ZCCHC11 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ZCCHC11 antibody detects endogenous levels of ZCCHC11.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1645
Applicable Applications for anti-ZCCHC11 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ZCCHC11.
Subcellular Location
Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS.
Predicted Cross Reactivity
Chicken
Similarity
Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of MCF7 cells, using ZCCHC11 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of MCF7 cells, using ZCCHC11 antibody.)
IHC (Immunohiostchemistry)
(AAA323007 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA323007 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using ZCCHC11 antibody. Lane 1: hepg2 treated with blocking peptide. Lane 2: Hepg2; Lane 3: B16F10;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using ZCCHC11 antibody. Lane 1: hepg2 treated with blocking peptide. Lane 2: Hepg2; Lane 3: B16F10;)
Related Product Information for anti-ZCCHC11 antibody
Function: Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:25480299). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity). Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A. Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (By similarity). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.
Subunit Structure: Interacts with LIN28A in the presence of pre-let-7 RNA. Interacts with T2BP.
Similarity: Belongs to the DNA polymerase type-B-like family.
Subunit Structure: Interacts with LIN28A in the presence of pre-let-7 RNA. Interacts with T2BP.
Similarity: Belongs to the DNA polymerase type-B-like family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 185 kDa
Predicted: 186 kDa
Predicted: 186 kDa
NCBI Official Full Name
terminal uridylyltransferase 4 isoform a
NCBI Official Synonym Full Names
terminal uridylyl transferase 4
NCBI Official Symbol
TUT4
NCBI Official Synonym Symbols
PAPD3; TENT3A; ZCCHC11
NCBI Protein Information
terminal uridylyltransferase 4
UniProt Protein Name
Terminal uridylyltransferase 4
UniProt Gene Name
TUT4
UniProt Synonym Gene Names
TUTase 4
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Product Notes
The ZCCHC11 tut4 (Catalog #AAA323007) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZCCHC11 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZCCHC11 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ZCCHC11 tut4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZCCHC11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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