FCM/FACS (Flow Cytometry)
(ZCRB1 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ZCRB1 Polyclonal Antibody | anti-ZCRB1 antibody
ZCRB1 Antibody (C-term)
Gene Names
ZCRB1; MADP1; RBM36; MADP-1; SNRNP31; ZCCHC19
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Xenopus)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ZCRB1, Antibody; ZCRB1 Antibody (C-term); Zinc finger CCHC-type and RNA-binding motif-containing protein 1; U11/U12 small nuclear ribonucleoprotein 31 kDa protein; U11/U12 snRNP 31 kDa protein; U11/U12-31K; ZCRB1; anti-ZCRB1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ZCRB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the C-terminal region of human ZCRB1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
184-212
Sequence Length
217
Applicable Applications for anti-ZCRB1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ZCRB1 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ZCRB1 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(ZCRB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZCRB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ZCRB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZCRB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ZCRB1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the ZCRB1 antibody detected the ZCRB1 protein (arrow).)
WB (Western Blot)
(ZCRB1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the ZCRB1 antibody detected the ZCRB1 protein (arrow).)
Related Product Information for anti-ZCRB1 antibody
Pre-mRNA splicing is catalyzed by the spliceosome.
U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5'
splice site and branch-point sequence. U11 and U12 snRNPs are
components of U12-type spliceosome and function as a molecular
bridge connecting both ends of the intron. The protein encoded by
this gene contains a RNA recognition motif. It was identified as
one of the protein components of U11/U12 snRNPs. This protein and
many other U11/U12 snRNP proteins are highly conserved in organisms
known to contain U12-type introns. These proteins have been shown
to be essential for cell viability, suggesting the key roles in
U12-type splicing.
U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5'
splice site and branch-point sequence. U11 and U12 snRNPs are
components of U12-type spliceosome and function as a molecular
bridge connecting both ends of the intron. The protein encoded by
this gene contains a RNA recognition motif. It was identified as
one of the protein components of U11/U12 snRNPs. This protein and
many other U11/U12 snRNP proteins are highly conserved in organisms
known to contain U12-type introns. These proteins have been shown
to be essential for cell viability, suggesting the key roles in
U12-type splicing.
References
Wang, H., et al. Genomics 89(1):59-69(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Will, C.L., et al. RNA 10(6):929-941(2004)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Will, C.L., et al. RNA 10(6):929-941(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24592
NCBI Official Full Name
zinc finger CCHC-type and RNA-binding motif-containing protein 1
NCBI Official Synonym Full Names
zinc finger CCHC-type and RNA binding motif 1
NCBI Official Symbol
ZCRB1
NCBI Official Synonym Symbols
MADP1; RBM36; MADP-1; SNRNP31; ZCCHC19
NCBI Protein Information
zinc finger CCHC-type and RNA-binding motif-containing protein 1
UniProt Protein Name
Zinc finger CCHC-type and RNA-binding motif-containing protein 1
UniProt Gene Name
ZCRB1
UniProt Synonym Gene Names
U11/U12 snRNP 31 kDa protein; U11/U12-31K
UniProt Entry Name
ZCRB1_HUMAN
Similar Products
Product Notes
The ZCRB1 zcrb1 (Catalog #AAA285745) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 184-212. The ZCRB1 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's ZCRB1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ZCRB1 zcrb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZCRB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.