Rabbit anti-Human ZDHHC3 Polyclonal Antibody | anti-ZDHHC3 antibody

ZDHHC3 Antibody

Cat. Num. AAA230010

• Gene Names: ZDHHC3; GODZ; DHHC-3; ZNF373
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: ZDHHC3, Antibody; ZDHHC3 Antibody; Palmitoyltransferase ZDHHC3Curated (EC:2.3.1.2251 Publication; ZDHHC3; Protein DHHC1; anti-ZDHHC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Positions: 94-171

• Applicable Applications for anti-ZDHHC3 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Application Notes: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:100
• Immunogen: Recombinant Human Palmitoyltransferase ZDHHC3 protein (94-171AA)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

WB (Western Blot)

(Positive WB detected in: Hela whole cell lysate(20ug),K562 whole cell lysate(20ug), JK whole cell lysate(20ug), U251 whole cell lysate(20ug), HepG2 whole cell lysate(20ug), MCF7 whole cell lysate(20ugAll lanes: ZDHHC3 antibody at 1:1000Secondary: Goat polyclonal to rabbit IgG at 1/20000 dilutionPredicted band size: 35,38 kDaObserved band size: 35 kDaExposure time: 60s)

IHC (Immunohistochemistry)

(IHC image diluted at 1:50 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and 2 visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody)

Related Product Information for anti-ZDHHC3 antibody

Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi. May also palmitoylate DLG4, DNAJC5 and SNAP25.

Product Categories/Family for anti-ZDHHC3 antibody

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 206597477
• NCBI GeneID: 51304
• NCBI Accession #: NP_001128651.1
• NCBI GenBank Nucleotide #: NM_001135179.1
• UniProt Accession #: Q9NYG2; Q53A17; Q96BL0
• NCBI Official Full Name: palmitoyltransferase ZDHHC3 isoform 1
• NCBI Official Synonym Full Names: zinc finger DHHC-type containing 3
• NCBI Official Symbol: ZDHHC3
• NCBI Official Synonym Symbols: GODZ; DHHC-3; ZNF373
• NCBI Protein Information: palmitoyltransferase ZDHHC3
• UniProt Protein Name: Palmitoyltransferase ZDHHC3
• UniProt Gene Name: ZDHHC3
• UniProt Synonym Gene Names: DHHC-3
• UniProt Entry Name: ZDHC3_HUMAN