FCM/FACS (Flow Cytometry)
(ZEB2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ZEB2 Polyclonal Antibody | anti-ZEB2 antibody
ZEB2 Antibody (C-term)
Gene Names
ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ZEB2, Antibody; ZEB2 Antibody (C-term); Zinc finger E-box-binding homeobox 2; Smad-interacting protein 1; SMADIP1; Zinc finger homeobox protein 1b; ZEB2; KIAA0569; SIP1; ZFHX1B; ZFX1B; anti-ZEB2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ZEB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1078-1105 amino acids from the C-terminal region of human ZEB2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.35 (varies by lot)
Sequence Positions
1078-1105
Sequence Length
1190
Applicable Applications for anti-ZEB2 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ZEB2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ZEB2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(ZEB2 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ZEB2 antibody detected the ZEB2 protein (arrow).)
WB (Western Blot)
(ZEB2 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ZEB2 antibody detected the ZEB2 protein (arrow).)
Related Product Information for anti-ZEB2 antibody
The protein encoded by this gene is a member of the Zfh1
family of 2-handed zinc finger/homeodomain proteins. It is located
in the nucleus and functions as a DNA-binding transcriptional
repressor that interacts with activated SMADs. Mutations in this
gene are associated with Hirschsprung disease/Mowat-Wilson
syndrome. Alternatively spliced transcript variants have been found
for this gene.
family of 2-handed zinc finger/homeodomain proteins. It is located
in the nucleus and functions as a DNA-binding transcriptional
repressor that interacts with activated SMADs. Mutations in this
gene are associated with Hirschsprung disease/Mowat-Wilson
syndrome. Alternatively spliced transcript variants have been found
for this gene.
Product Categories/Family for anti-ZEB2 antibody
References
Kumar, P.A., et al. J. Biol. Chem. 285(41):31148-31156(2010)
Li, A., et al. Cancer Res. 70(13):5226-5237(2010)
Ohashi, S., et al. Cancer Res. 70(10):4174-4184(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Eriksson, N., et al. PLoS Genet. 6 (6), E1000993 (2010) :
Li, A., et al. Cancer Res. 70(13):5226-5237(2010)
Ohashi, S., et al. Cancer Res. 70(10):4174-4184(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Eriksson, N., et al. PLoS Genet. 6 (6), E1000993 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
136447
NCBI Official Full Name
zinc finger E-box-binding homeobox 2 isoform 2
NCBI Official Synonym Full Names
zinc finger E-box binding homeobox 2
NCBI Official Symbol
ZEB2
NCBI Official Synonym Symbols
SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
NCBI Protein Information
zinc finger E-box-binding homeobox 2
UniProt Protein Name
Zinc finger E-box-binding homeobox 2
UniProt Gene Name
ZEB2
UniProt Synonym Gene Names
KIAA0569; SIP1; ZFHX1B; ZFX1B; SMADIP1
UniProt Entry Name
ZEB2_HUMAN
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Product Notes
The ZEB2 zeb2 (Catalog #AAA285674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1078-1105. The ZEB2 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's ZEB2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ZEB2 zeb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZEB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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