WB (Western Blot)
(All lanes : Anti-ZFYVE20 Antibody (N-Term) at 1:8000 dilutionLane 1: Hela whole cell lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human ZFYVE20 Polyclonal Antibody | anti-RBSN antibody
ZFYVE20 Antibody (N-Term)
Gene Names
RBSN; ZFYVE20; Rabenosyn-5
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ZFYVE20, Antibody; ZFYVE20 Antibody (N-Term); Rabenosyn-5; 110 kDa protein; FYVE finger-containing Rab5 effector protein rabenosyn-5; RAB effector RBSN {ECO:0000312|HGNC:HGNC:20759}; Zinc finger FYVE domain-containing protein 20; RBSN HGNC:20759); anti-RBSN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ZFYVE20 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 41-65 amino acids from the human region of human ZFYVE20.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
784
Applicable Applications for anti-RBSN antibody
ELISA, WB (Western Blot)
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-ZFYVE20 Antibody (N-Term) at 1:8000 dilutionLane 1: Hela whole cell lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-ZFYVE20 Antibody (N-Term) at 1:8000 dilutionLane 1: Hela whole cell lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-ZFYVE20 Antibody (N-Term)at 1:2000 dilution + A431 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-ZFYVE20 Antibody (N-Term)at 1:2000 dilution + A431 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RBSN antibody
Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3) (PubMed:11062261, PubMed:11788822, PubMed:15020713). Plays a role in the recycling of transferrin receptor to the plasma membrane (PubMed:22308388).
Product Categories/Family for anti-RBSN antibody
References
Nielsen E.,et al.J. Cell Biol. 151:601-612(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
de Renzis S.,et al.Nat. Cell Biol. 4:124-133(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
de Renzis S.,et al.Nat. Cell Biol. 4:124-133(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88870
NCBI Official Full Name
rabenosyn-5
NCBI Official Synonym Full Names
rabenosyn, RAB effector
NCBI Official Symbol
RBSN
NCBI Official Synonym Symbols
ZFYVE20; Rabenosyn-5
NCBI Protein Information
rabenosyn-5
UniProt Protein Name
Rabenosyn-5
UniProt Gene Name
RBSN
UniProt Entry Name
RBNS5_HUMAN
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Product Notes
The RBSN rbsn (Catalog #AAA286010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZFYVE20 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZFYVE20 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RBSN rbsn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZFYVE20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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