Rabbit ZIF268 / EGR1 Polyclonal Antibody | anti-ZIF268 antibody

Rabbit Polyclonal to Human ZIF268 / EGR1

Cat. Num. AAA50753

• Gene Names: EGR1; TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268
• Reactivity: Human, Mouse; Predicted: Chimpanzee
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: ZIF268 / EGR1, Antibody; Rabbit Polyclonal to Human ZIF268 / EGR1; Anti-ZIF268 / EGR1 Antibody (aa94-108) IHC-plus; EGR1; AT225; Early growth response 1; G0S30; KROX24; NGFIA; KROX-24; TIS8; ZIF-268; ZIF268; Zinc finger protein Krox-24; EGR-1; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Human ZIF268; anti-ZIF268 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Chimpanzee
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1.
• Purity/Purification: Affinity Purified
• Form/Format: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Concentration: 0.93 mg/ml (varies by lot)
• Sequence Length: 543

• Applicable Applications for anti-ZIF268 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human EGR1.
• Immunogen Type: Synthetic peptide
• Immunogen: ZIF268 / EGR1 antibody was raised against this affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 85-115 of Human EGR-1
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C or -20°C, Avoid repeated freezing and thawing.

WB (Western Blot)

(Anti-EGR-1 Antibody - Western Blot. Western blot of Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kD corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Reaction occurred 2 hours at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)

IHC (Immunohistochemistry)

(Anti-EGR1 antibody IHC of human lung small cell carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503493
• NCBI GeneID: 1958
• NCBI Accession #: NP_001955.1
• NCBI GenBank Nucleotide #: NM_001964.2
• UniProt Accession #: P18146
• NCBI Official Full Name: early growth response protein 1
• NCBI Official Synonym Full Names: early growth response 1
• NCBI Official Symbol: EGR1
• NCBI Official Synonym Symbols: TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268
• NCBI Protein Information: early growth response protein 1; EGR-1; zinc finger protein 225; transcription factor ETR103; transcription factor Zif268; zinc finger protein Krox-24; nerve growth factor-induced protein A
• UniProt Protein Name: Early growth response protein 1
• UniProt Gene Name: EGR1
• UniProt Synonym Gene Names: KROX24; ZNF225; EGR-1; NGFI-A
• UniProt Entry Name: EGR1_HUMAN