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IP (Immunoprecipitation)
(AZGP1 was immunoprecipitated using:Lane A:0.5 mg Colo-205 Whole Cell Lysate2 uL anti-AZGP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AZGP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :43 kDa)
Rabbit anti-Human Zinc Alpha 2 Glycoprotein/AZGP1 Polyclonal Antibody | anti-AZGP1 antibody
Anti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody, Rabbit Polyclonal
Gene Names
AZGP1; ZAG; ZA2G
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Zinc Alpha 2 Glycoprotein/AZGP1, Antibody; Anti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody, Rabbit Polyclonal; Zinc Alpha 2 Glycoprotein Antibody, Rabbit PAb, Antigen Affinity Purified; alpha-2-glycoprotein 1, zinc-binding; Anti-ZA2G Antibody; Anti-ZAG Antibody; anti-AZGP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Zinc Alpha 2 Glycoprotein
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-AZGP1 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human Zinc Alpha 2 Glycoprotein Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Zinc Alpha 2 Glycoprotein (rh Zinc Alpha 2 Glycoprotein). Zinc Alpha 2 Glycoprotein specific IgG was purified by Human Zinc Alpha 2 Glycoprotein affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(AZGP1 was immunoprecipitated using:Lane A:0.5 mg Colo-205 Whole Cell Lysate2 uL anti-AZGP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AZGP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :43 kDa)
IP (Immunoprecipitation)
(AZGP1 was immunoprecipitated using:Lane A:0.5 mg Colo-205 Whole Cell Lysate2 uL anti-AZGP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AZGP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :43 kDa)
IHC (Immunohistochemisry)
(Immunochemical staining of human AZGP1 in human prostate with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human AZGP1 in human prostate with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human AZGP1 in human liver with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human AZGP1 in human liver with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-AZGP1 rabbit polyclonal antibody at 1:500 dilutionLane A: COLO205 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:44 kDa)
WB (Western Blot)
(Anti-AZGP1 rabbit polyclonal antibody at 1:500 dilutionLane A: COLO205 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:44 kDa)
Related Product Information for anti-AZGP1 antibody
Alpha-2-glycoprotein, also known as AZGP1, belongs to the MHC class I family. It can be detected in body fluids such as serum, sweat, and seminal and breast cyst fluids. It has been shown that alpha-2-glycoprotein can stimulate lipolysis by adipocytes in vivo and in vitro. Thus it is believed that alpha-2-glycoprotein plays an important role in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and it also regulates melanin production by normal and malignant melanocytes. Alpha-2-glycoprotein is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia.
References
Tada T, et al. (1991) Immunohistochemical localization of Zn-alpha 2-glycoprotein in normal human tissues. J Histochem Cytochem. 39(9):1221-6.Vanni H, et al. (2009) Cigarette Smoking Induces Overexpression of a Fat-Depleting Gene AZGP1 in the Human. Chest. 135(5):1197-208.Ueyama H, et al. (1991) Cloning and nucleotide sequence of a human Zn-alpha 2-glycoprotein cDNA and chromosomal assignment of its gene. Biochem Biophys Res Commun. 177(2):696-703.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,259 Da
NCBI Official Full Name
zinc-alpha-2-glycoprotein
NCBI Official Synonym Full Names
alpha-2-glycoprotein 1, zinc-binding
NCBI Official Symbol
AZGP1
NCBI Official Synonym Symbols
ZAG; ZA2G
NCBI Protein Information
zinc-alpha-2-glycoprotein; zn-alpha-2-GP; Zn-alpha2-glycoprotein; zn-alpha-2-glycoprotein; Alpha-2-glycoprotein, zinc
UniProt Protein Name
Zinc-alpha-2-glycoprotein
UniProt Gene Name
AZGP1
UniProt Synonym Gene Names
ZAG; ZNGP1; Zn-alpha-2-GP
UniProt Entry Name
ZA2G_HUMAN
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Product Notes
The AZGP1 azgp1 (Catalog #AAA255662) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Zinc Alpha 2 Glycoprotein/AZGP1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AZGP1 azgp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zinc Alpha 2 Glycoprotein/AZGP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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