IP (Immunoprecipitation)
(Detection of human ZNF295 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF295 antibody AAA211518 used for IP at 3 ug/mg lysate. ZNF295 was also immunoprecipitated by rabbit anti-ZNF295 antibody which recognizes a downstream epitope. For blotting immunoprecipitated ZNF295, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human ZNF295 Polyclonal Antibody | anti-ZNF295 antibody
Rabbit anti-ZNF295 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
ZNF295, Antibody; Rabbit anti-ZNF295 Antibody, Affinity Purified; zinc finger and BTB domain-containing protein 21; zinc finger protein 295; ZNF295; Zinc finger and BTB domain-containing protein 21; zinc finger and BTB domain containing 21; anti-ZNF295 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ZNF295 antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 875 and 925
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human ZNF295 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF295 antibody AAA211518 used for IP at 3 ug/mg lysate. ZNF295 was also immunoprecipitated by rabbit anti-ZNF295 antibody which recognizes a downstream epitope. For blotting immunoprecipitated ZNF295, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ZNF295 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF295 antibody AAA211518 used for IP at 3 ug/mg lysate. ZNF295 was also immunoprecipitated by rabbit anti-ZNF295 antibody which recognizes a downstream epitope. For blotting immunoprecipitated ZNF295, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human ZNF295 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZNF295 (Cat. No. AAA211518 Lot1) used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100)
IHC (Immunohistochemistry)
(Detection of human ZNF295 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZNF295 (Cat. No. AAA211518 Lot1) used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100)
Related Product Information for anti-ZNF295 antibody
ZNF295 bears 8 C2H2-type zinc fingers and a BTB/POZ (broad-complex, tramtrack, bric a brac/Pox-virus and zinc finger) domain indicating a function in transcriptional regulation. ZNF295 has been shown to function as a transcriptional repressor and associates with ZFP161, a zinc-finger protein also know to function as a repressor of transcription.
Product Categories/Family for anti-ZNF295 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ZNF295 zbtb21 (Catalog #AAA211518) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ZNF295 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF295 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the ZNF295 zbtb21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF295, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.