IF (Immunofluorescence)
(Immunofluorescence of ZNF521 in HeLa cells with ZNF521 antibody at 20 μg/mL.)
Rabbit anti-Human ZNF521 Polyclonal Antibody | anti-ZNF521 antibody
ZNF521 Antibody
Gene Names
ZNF521; EHZF; Evi3
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
ZNF521 Antibody is affinity chromatography purified via peptide column.
Synonyms
ZNF521, Antibody; ZNF521 Antibody; ZNF521; EHZF; Evi3; LIP3; Zinc finger protein 521; Early hematopoietic zinc finger protein; zinc finger protein 521; anti-ZNF521 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ZNF521 antibody is human specific.
Purity/Purification
ZNF521 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1311
Applicable Applications for anti-ZNF521 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
ZNF521 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521.
Buffer
ZNF521 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ZNF521 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Immunofluorescence of ZNF521 in HeLa cells with ZNF521 antibody at 20 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of ZNF521 in HeLa cells with ZNF521 antibody at 20 μg/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of ZNF521 in HeLa cells with ZNF521 antibody at 2.5 μg/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of ZNF521 in HeLa cells with ZNF521 antibody at 2.5 μg/mL.)
WB (Western Blot)
(Western blot analysis of ZNF521 in HeLa cell lysate with ZNF521 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
WB (Western Blot)
(Western blot analysis of ZNF521 in HeLa cell lysate with ZNF521 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Related Product Information for anti-ZNF521 antibody
ZNF521 Antibody: The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
147,866 Da
NCBI Official Full Name
zinc finger protein 521
NCBI Official Synonym Full Names
zinc finger protein 521
NCBI Official Symbol
ZNF521
NCBI Official Synonym Symbols
EHZF; Evi3
NCBI Protein Information
zinc finger protein 521; LYST-interacting protein 3; early hematopoietic zinc finger protein
UniProt Protein Name
Zinc finger protein 521
UniProt Gene Name
ZNF521
UniProt Synonym Gene Names
EHZF; LIP3
UniProt Entry Name
ZN521_HUMAN
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Product Notes
The ZNF521 znf521 (Catalog #AAA39532) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZNF521 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF521 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ZNF521 znf521 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF521, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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