WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and ZNF566 knockdown (KD) 293T cells using ZNF566 Rabbit pAb (AAA283217) at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Rabbit anti-Human ZNF566 Polyclonal Antibody | anti-ZNF566 antibody
ZNF566 Rabbit pAb
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
ZNF566, Antibody; ZNF566 Rabbit pAb; ZNF566; zinc finger protein 566; 66; anti-ZNF566 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Sequence
SKPNVISYLEQGKEPWLADRELTRGQWPVLESRCETKKLFLKKEIYEIESTQWEIMEKLTRRDFQCSSFRDDWECNRQFKKELGSQGGHFNQLVFTHEDLPTLSH
Applicable Applications for anti-ZNF566 antibody
ELISA, WB (Western Blot)
Immunogen
Recombinant Protein corresponding to a sequence within amino acids 50-154 of human ZNF566(NP_001138816.1).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and ZNF566 knockdown (KD) 293T cells using ZNF566 Rabbit pAb (AAA283217) at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and ZNF566 knockdown (KD) 293T cells using ZNF566 Rabbit pAb (AAA283217) at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of lysates from SH-SY5Y cells, using [KO Validated] ZNF566 Rabbit pAb (AAA283217) at 1:800 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of lysates from SH-SY5Y cells, using [KO Validated] ZNF566 Rabbit pAb (AAA283217) at 1:800 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Related Product Information for anti-ZNF566 antibody
Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II. Predicted to be located in nucleus. Predicted to be integral component of membrane.
Product Categories/Family for anti-ZNF566 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 49kDa
Observed MW: 56kDa/49kDa
Observed MW: 56kDa/49kDa
UniProt Protein Name
Zinc finger protein 566
UniProt Gene Name
ZNF566
UniProt Entry Name
ZN566_HUMAN
Similar Products
Product Notes
The ZNF566 znf566 (Catalog #AAA283217) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZNF566 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF566 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ZNF566 znf566 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SKPNVISYLE QGKEPWLADR ELTRGQWPVL ESRCETKKLF LKKEIYEIES TQWEIMEKLT RRDFQCSSFR DDWECNRQFK KELGSQGGHF NQLVFTHEDL PTLSH. It is sometimes possible for the material contained within the vial of "ZNF566, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.