IF (Immunofluorescence)
((10ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1: 100).)
Goat ZNRF1 Polyclonal Antibody | anti-Znrf1 antibody
Goat anti-ZNRF1 (mouse) Antibody
Gene Names
Znrf1; Rnf42; Zrfp1; nin283; B830022L21Rik
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ZNRF1, Antibody; Goat anti-ZNRF1 (mouse) Antibody; Znrf1; zinc and ring finger 1; B830022L21Rik; MGC101991; Rnf42; Zrfp1; nin283; E3 ubiquitin-protein ligase ZNRF1; nerve injury-induced gene 283 protein; peripheral nerve injury protein nin283; ring finger protein 42; zinc ring finger protein 1; zinc/RING; B830022L21Rik antibody; E3 ubiquitin-protein ligase ZNRF1 antibody; MGC101991 antibody; nerve injury-induced gene 283 protein antibody; nin283 antibody; peripheral nerve injury protein nin283 antibody; ring finger protein 42 antibody; Rnf42 antibody; zinc and ring finger 1 antibody; zinc ring finger protein 1 antibody; zinc/RING finger protein 1 antibody; Znrf1 antibody; Zrfp1 antibody; ZNRF1 (mouse); anti-Znrf1 antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SDSTYAHGNGYQET
Sequence Length
227
Applicable Applications for anti-Znrf1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-SDSTYAHGNGYQET, from the internal region of the protein sequence according to NP_573469.1; NP_001162093.1.
Epitope
Internal region
Note
This antibody is expected to recognize reported isoforms a and b (NP_573469.1; NP_001162093.1). Reported variants represent identical protein: NP_001162092.1, NP_573469.1
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IF (Immunofluorescence)
((10ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1: 100).)
IF (Immunofluorescence)
((10ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1: 100).)
WB (Western Blot)
(N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with (1ug/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with (1ug/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,843 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase ZNRF1 isoform a
NCBI Official Synonym Full Names
zinc and ring finger 1
NCBI Official Symbol
Znrf1
NCBI Official Synonym Symbols
Rnf42; Zrfp1; nin283; B830022L21Rik
NCBI Protein Information
E3 ubiquitin-protein ligase ZNRF1
UniProt Protein Name
E3 ubiquitin-protein ligase ZNRF1
UniProt Gene Name
Znrf1
UniProt Synonym Gene Names
Nin283
UniProt Entry Name
ZNRF1_MOUSE
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Product Notes
The Znrf1 znrf1 (Catalog #AAA61659) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ZNRF1 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ZNRF1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the Znrf1 znrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SDSTYAHGNG YQET. It is sometimes possible for the material contained within the vial of "ZNRF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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