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IHC (Immunohistochemistry)
(IHC of Mouse kidney using Z01 antibody and FFPE tissue after heat-induced retrieval. Anti-Z01 Ab at 1:750/DAB detection.)
Goat ZO-1 Polyclonal Antibody | anti-ZO-1 antibody
ZO-1 Polyclonal Antibody
Gene Names
Tjp1; ZO1
Reactivity
Reacts against Human, Rat, Mouse, Canine and Monkey proteins.
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Epitope-affinity purified from goat antiserum
Synonyms
ZO-1, Antibody; ZO-1 Polyclonal Antibody; ZO-1 and zona occludens protein 1 antibody; anti-ZO-1 antibody
Host
Goat
Reactivity
Reacts against Human, Rat, Mouse, Canine and Monkey proteins.
Clonality
Polyclonal
Specificity
Detects endogenous levels of total ZO-1 by Western blot in whole cell and tissue lysates.
Purity/Purification
Epitope-affinity purified from goat antiserum
Form/Format
Polyclonal antibody supplied as a 200 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Applicable Applications for anti-ZO-1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) - Frozen/Paraffin
Application Notes
Western blot: 1:500-1:2,000
lmmunofluorescence: 1:50-1:250
Immunohistochemistry (paraffin): 1:200-1:1000
Immunohistochemistry (frozen): 1:200-1:1000
Special instructions: The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles..
lmmunofluorescence: 1:50-1:250
Immunohistochemistry (paraffin): 1:200-1:1000
Immunohistochemistry (frozen): 1:200-1:1000
Special instructions: The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles..
Immunogen
Purified recombinant peptide derived from within residues 1580 aa to the C-terminus of human ZO-1 produced in E. coli.
Preparation and Storage
For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.
IHC (Immunohistochemistry)
(IHC of Mouse kidney using Z01 antibody and FFPE tissue after heat-induced retrieval. Anti-Z01 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of Mouse kidney using Z01 antibody and FFPE tissue after heat-induced retrieval. Anti-Z01 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.)
IHC (Immunohistchemistry)
(IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.)
IHC (Immunohistchemistry)
(IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with ZO1 Ab at 1/100 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with ZO1 Ab at 1/100 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)
IF (Immunofluorescence)
(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)
WB (Western Blot)
(Anti-ZO1 Ab at 1/2,500 dilution; 50ug of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.)
WB (Western Blot)
(Anti-ZO1 Ab at 1/2,500 dilution; 50ug of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.)
Immunoperoxidase
(Immunoperoxidase of polyclonal antibody to ZO-1 (1/200) on paraformaldehyde-fixed paraffin-embedded mouse kidney.)
Immunoperoxidase
(Immunoperoxidase of polyclonal antibody to ZO-1 (1/200) on paraformaldehyde-fixed paraffin-embedded mouse kidney.)
Reactivity Chart
Reactivity Chart
Related Product Information for anti-ZO-1 antibody
Goat polyclonal to TJPl tight Junction protein 1 (TJP1). TJPl is located on a cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
ZO-1
NCBI Official Synonym Full Names
tight junction protein 1
NCBI Official Symbol
Tjp1
NCBI Official Synonym Symbols
ZO1
NCBI Protein Information
tight junction protein ZO-1; zona occludens protein 1; zonula occludens protein 1
UniProt Protein Name
Tight junction protein ZO-1
UniProt Gene Name
Tjp1
UniProt Synonym Gene Names
Zo1
UniProt Entry Name
ZO1_MOUSE
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Product Notes
The ZO-1 tjp1 (Catalog #AAA13861) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The ZO-1 Polyclonal Antibody reacts with Reacts against Human, Rat, Mouse, Canine and Monkey proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's ZO-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) - Frozen/Paraffin. Western blot: 1:500-1:2,000 lmmunofluorescence: 1:50-1:250 Immunohistochemistry (paraffin): 1:200-1:1000 Immunohistochemistry (frozen): 1:200-1:1000 Special instructions: The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.. Researchers should empirically determine the suitability of the ZO-1 tjp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZO-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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