WB (Western Blot)
(Western blot analysis of ZO-1 using various lysatesLanes 1 - 2: Merged signal (red and green). Green - AAA31075 observed at 195 kDa. Red - loading control, , observed at 55 kDa. Blots were developed with Goat Anti- Rabbit IgG(H+L) FITC–conjugated and Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated secondary antibodies)
Rabbit ZO 1 Polyclonal Antibody | anti-ZO-1 antibody
ZO 1 Antibody
Gene Names
TJP1; ZO-1
Reactivity
Human, Mouse, Rat, Pig, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
ZO 1, Antibody; ZO 1 Antibody; Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1; anti-ZO-1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig, Monkey
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Sequence Length
1748
Applicable Applications for anti-ZO-1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
The optimal dilutions to be determined by the end user.
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
The optimal dilutions to be determined by the end user.
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human ZO 1, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of ZO-1 using various lysatesLanes 1 - 2: Merged signal (red and green). Green - AAA31075 observed at 195 kDa. Red - loading control, , observed at 55 kDa. Blots were developed with Goat Anti- Rabbit IgG(H+L) FITC–conjugated and Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated secondary antibodies)
WB (Western Blot)
(Western blot analysis of ZO-1 using various lysatesLanes 1 - 2: Merged signal (red and green). Green - AAA31075 observed at 195 kDa. Red - loading control, , observed at 55 kDa. Blots were developed with Goat Anti- Rabbit IgG(H+L) FITC–conjugated and Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated secondary antibodies)
Related Product Information for anti-ZO-1 antibody
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
References
Hui-Fang Wang, Jing-XueMa, Qing-LiShang, Jian-BinAn, Hai-TingChen; Journal: EUR J PHARMACOL. Crocetin inhibits the proliferation, migration and TGF-beta 2-induced epithelial-mesenchymal transition of retinal pigment epithelial cells. Wang J, Lu J, Xie X, Xiong J, Huang N, Wei H, Jiang S, Peng J; Journal: Int Immunopharmacol. Blend of organic acids and medium chain fatty acids prevents the inflammatory response and intestinal barrier dysfunction in mice challenged with enterohemorrhagic Escherichia coli O157:H7. Zou Y, Xiang Q, Wang J, Peng J, Wei H; Journal: Biomed Res Int. Oregano Essential Oil Improves Intestinal Morphology and Expression of Tight Junction Proteins Associated with Modulation of Selected Intestinal Bacteria and Immune Status in a Pig Model.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
195 kDa
NCBI Official Full Name
tight junction protein ZO-1 isoform a
NCBI Official Synonym Full Names
tight junction protein 1
NCBI Official Symbol
TJP1
NCBI Official Synonym Symbols
ZO-1
NCBI Protein Information
tight junction protein ZO-1
UniProt Protein Name
Tight junction protein ZO-1
UniProt Gene Name
TJP1
UniProt Synonym Gene Names
ZO1
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Product Notes
The ZO-1 tjp1 (Catalog #AAA31075) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZO 1 Antibody reacts with Human, Mouse, Rat, Pig, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ZO 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500 The optimal dilutions to be determined by the end user. For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the ZO-1 tjp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZO 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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