WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ZO1 antibody)
Rabbit anti-Human ZO1 Polyclonal Antibody | anti-ZO1 antibody
ZO1
Gene Names
TJP1; ZO-1
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Synonyms
ZO1, Antibody; ZO1; anti-ZO1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
Affinity Purified antibody
PBS with 0.02% sodium azide, 50% glycerol, pH7.4. 100ul aliquot
PBS with 0.02% sodium azide, 50% glycerol, pH7.4. 100ul aliquot
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ZO1 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Antibody can also be aliquoted and stored frozen at -20 degree C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ZO1 antibody)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ZO1 antibody)
IHC (Immunohistochemistry)
(Immunohistochemical stain of paraffin-embedded human kidney using ZO1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemical stain of paraffin-embedded human kidney using ZO1 antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-ZO1 antibody
Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton. ZO-1 and -2 are required for tight junction formation and function. In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators. The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
1748
NCBI Official Full Name
Tight junction protein ZO-1
NCBI Official Synonym Full Names
tight junction protein 1
NCBI Official Symbol
TJP1
NCBI Official Synonym Symbols
ZO-1
NCBI Protein Information
tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein
UniProt Protein Name
Tight junction protein ZO-1
UniProt Gene Name
TJP1
UniProt Synonym Gene Names
ZO1
UniProt Entry Name
ZO1_HUMAN
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Product Notes
The ZO1 tjp1 (Catalog #AAA76698) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZO1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZO1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ZO1 tjp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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