IHC (Immunohiostchemistry)
(ZP4 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human ZP4 Polyclonal Antibody | anti-ZP4 antibody
ZP4 Antibody (C-term)
Gene Names
ZP4; ZBP; ZP1; ZPB; Zp-4
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ZP4, Antibody; ZP4 Antibody (C-term); Zona pellucida sperm-binding protein 4; Zona pellucida glycoprotein 4; Zp-4; Zona pellucida protein B; Processed zona pellucida sperm-binding protein 4; ZP4; ZPB; anti-ZP4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ZP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human ZP4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
445-474
Sequence Length
540
Applicable Applications for anti-ZP4 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(ZP4 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ZP4 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ZP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).)
WB (Western Blot)
(ZP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).)
Related Product Information for anti-ZP4 antibody
The zona pellucida is an extracellular matrix that
surrounds the oocyte and early embryo. It is composed primarily of
three or four glycoproteins with various functions during
fertilization and preimplantation development. The nascent protein
contains a N-terminal signal peptide sequence, a conserved ZP
domain, a consensus furin cleavage site, and a C-terminal
transmembrane domain. It is hypothesized that furin cleavage
results in release of the mature protein from the plasma membrane
for subsequent incorporation into the zona pellucida matrix.
However, the requirement for furin cleavage in this process remains
controversial based on mouse studies. Previously, this gene has
been referred to as ZP1 or ZPB and thought to have similar
functions as mouse Zp1. However, a human gene with higher
similarity and chromosomal synteny to mouse Zp1 has been assigned
the symbol ZP1 and this gene has been assigned the symbol ZP4.
surrounds the oocyte and early embryo. It is composed primarily of
three or four glycoproteins with various functions during
fertilization and preimplantation development. The nascent protein
contains a N-terminal signal peptide sequence, a conserved ZP
domain, a consensus furin cleavage site, and a C-terminal
transmembrane domain. It is hypothesized that furin cleavage
results in release of the mature protein from the plasma membrane
for subsequent incorporation into the zona pellucida matrix.
However, the requirement for furin cleavage in this process remains
controversial based on mouse studies. Previously, this gene has
been referred to as ZP1 or ZPB and thought to have similar
functions as mouse Zp1. However, a human gene with higher
similarity and chromosomal synteny to mouse Zp1 has been assigned
the symbol ZP1 and this gene has been assigned the symbol ZP4.
Product Categories/Family for anti-ZP4 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
McCauley, J.L., et al. Genes Immun. 10(7):624-630(2009)
Nakano, M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(31):12838-12842(2009)
Choudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009)
Chiu, P.C., et al. Biol. Reprod. 79(5):869-877(2008)
McCauley, J.L., et al. Genes Immun. 10(7):624-630(2009)
Nakano, M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(31):12838-12842(2009)
Choudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009)
Chiu, P.C., et al. Biol. Reprod. 79(5):869-877(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59400
NCBI Official Full Name
zona pellucida sperm-binding protein 4 preproprotein
NCBI Official Synonym Full Names
zona pellucida glycoprotein 4
NCBI Official Symbol
ZP4
NCBI Official Synonym Symbols
ZBP; ZP1; ZPB; Zp-4
NCBI Protein Information
zona pellucida sperm-binding protein 4
UniProt Protein Name
Zona pellucida sperm-binding protein 4
UniProt Gene Name
ZP4
UniProt Synonym Gene Names
ZPB; Zp-4
UniProt Entry Name
ZP4_HUMAN
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Product Notes
The ZP4 zp4 (Catalog #AAA289198) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 445-474. The ZP4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZP4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ZP4 zp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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