WB (Western Blot)
(Detection of human ZWINT-1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ZWINT-1 antibody BL3453 (Cat. No. AAA211135) used for WB at 0.04 ug/ml (A) and at 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody BL3450 (Cat. No. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human ZWINT-1 Polyclonal Antibody | anti-ZWINT-1 antibody
Rabbit anti-ZWINT-1 Antibody, Affinity Purified
Gene Names
ZWINT; ZWINT1; KNTC2AP; HZwint-1
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ZWINT-1, Antibody; Rabbit anti-ZWINT-1 Antibody, Affinity Purified; HZwint-1; ZW10 interactor, kinetochore protein; zwint-1; ZWINT1; ZW10-interacting protein 1; SNAP25 interacting protein of 30 kDa; human ZW10 interacting protein-1; KNTC2AP; SIP30; ZW10 interactor; ZW10 interacting kinetochore protein; anti-ZWINT-1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ZWINT-1 antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 227 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ZWINT-1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ZWINT-1 antibody BL3453 (Cat. No. AAA211135) used for WB at 0.04 ug/ml (A) and at 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody BL3450 (Cat. No. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human ZWINT-1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ZWINT-1 antibody BL3453 (Cat. No. AAA211135) used for WB at 0.04 ug/ml (A) and at 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody BL3450 (Cat. No. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
ICC (Immunocytochemistry)
(Detection of human ZWINT-1 by immunocytochemistry. Sample: formaldehyde-fixed HeLa cells. Antibody: Affinity purified rabbit anti-ZWINT-1 (Cat. No. AAA211135 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated (Cat. No. used at a dilution of 1:100 (right).)
ICC (Immunocytochemistry)
(Detection of human ZWINT-1 by immunocytochemistry. Sample: formaldehyde-fixed HeLa cells. Antibody: Affinity purified rabbit anti-ZWINT-1 (Cat. No. AAA211135 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated (Cat. No. used at a dilution of 1:100 (right).)
Related Product Information for anti-ZWINT-1 antibody
Yeast 2-hybrid and localization studies show that human ZW10 interacting protein 1 (ZWINT-1) interacts with HZw10 in active centromeres and may play a role in directing HZw10 to the kinetochore during prometaphase. HZw10 is a component of the mitotic checkpoint that associates with the kinetochore to ensure chromosome fidelity during cell division.
Product Categories/Family for anti-ZWINT-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,544 Da
NCBI Official Full Name
ZW10 interactor isoform a
NCBI Official Synonym Full Names
ZW10 interacting kinetochore protein
NCBI Official Symbol
ZWINT
NCBI Official Synonym Symbols
ZWINT1; KNTC2AP; HZwint-1
NCBI Protein Information
ZW10 interactor; zwint-1; human ZW10 interacting protein-1; ZW10 interactor, kinetochore protein
UniProt Protein Name
ZW10 interactor
UniProt Gene Name
ZWINT
UniProt Synonym Gene Names
Zwint-1
UniProt Entry Name
ZWINT_HUMAN
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Product Notes
The ZWINT-1 zwint (Catalog #AAA211135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ZWINT-1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZWINT-1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ZWINT-1 zwint for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZWINT-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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