Standard Curve (Sample)
Porcine Chitinase-3-like Protein 1 (YKL-40/CHI3L1) ELISA Kit | CHI3L1 elisa kit
Porcine Chitinase-3-like Protein 1 (YKL-40/CHI3L1) ELISA Kit
Gene Names
CHI3L1; GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
Reactivity
Porcine
Synonyms
Chitinase-3-like Protein 1 (YKL-40/CHI3L1); N/A; Porcine Chitinase-3-like Protein 1 (YKL-40/CHI3L1) ELISA Kit; CHI3L1 elisa kit
Reactivity
Porcine
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CHI3L1 elisa kit
Intended Uses: This YARS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human YARS. This ELISA kit for research use only!
Principle of the Assay: YARS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-YARS antibody and an YARS-HRP conjugate. The assay sample and buffer are incubated together with YARS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YARS concentration since YARS from samples and YARS-HRP conjugate compete for the anti-YARS antibody binding site. Since the number of sites is limited, as more sites are occupied by YARS from the sample, fewer sites are left to bind YARS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YARS concentration in each sample is interpolated from this standard curve.
Principle of the Assay: YARS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-YARS antibody and an YARS-HRP conjugate. The assay sample and buffer are incubated together with YARS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YARS concentration since YARS from samples and YARS-HRP conjugate compete for the anti-YARS antibody binding site. Since the number of sites is limited, as more sites are occupied by YARS from the sample, fewer sites are left to bind YARS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YARS concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CHI3L1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,625 Da
NCBI Official Full Name
chitinase-3-like protein 1
NCBI Official Synonym Full Names
chitinase 3-like 1 (cartilage glycoprotein-39)
NCBI Official Symbol
CHI3L1
NCBI Official Synonym Symbols
GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
NCBI Protein Information
chitinase-3-like protein 1; 39 kDa synovial protein; cartilage glycoprotein 39
UniProt Protein Name
Chitinase-3-like protein 1
UniProt Gene Name
CHI3L1
UniProt Synonym Gene Names
CGP-39; GP-39; hCGP-39
UniProt Entry Name
CH3L1_HUMAN
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Product Notes
The Porcine CHI3L1 chi3l1 (Catalog #AAA245052) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245052 ELISA Kit recognizes Porcine CHI3L1. It is sometimes possible for the material contained within the vial of "Chitinase-3-like Protein 1 (YKL-40/CHI3L1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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