Porcine Glutathione S-Transferase Mu 3 ELISA Kit | GSTM3 elisa kit

Porcine Glutathione S-Transferase Mu 3 ELISA Kit

Cat. Num. AAA247419

• Gene Names: GSTM3; GST5; GSTB; GTM3; GSTM3-3
• Reactivity: Porcine
• Synonyms: Glutathione S-Transferase Mu 3; N/A; Porcine Glutathione S-Transferase Mu 3 ELISA Kit; GSTM3 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of GSTM3. No significant cross-reactivity or interference between GSTM3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GSTM3 and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 225
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Standard Curve (Sample)

Related Product Information for GSTM3 elisa kit

Principle of the Assay: GSTM3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GSTM3 antibody and an GSTM3-HRP conjugate. The assay sample and buffer are incubated together with GSTM3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSTM3 concentration since GSTM3 from samples and GSTM3-HRP conjugate compete for the anti-GSTM3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GSTM3 from the sample, fewer sites are left to bind GSTM3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSTM3 concentration in each sample is interpolated from this standard curve.

Intended Uses: This GSTM3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GSTM3. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 23065552
• NCBI GeneID: 2947
• NCBI Accession #: NP_000840.2
• NCBI GenBank Nucleotide #: NM_000849.4
• UniProt Accession #: P21266; O60550; Q96HA3
• Molecular Weight: 26,560 Da
• NCBI Official Full Name: glutathione S-transferase Mu 3
• NCBI Official Synonym Full Names: glutathione S-transferase mu 3
• NCBI Official Symbol: GSTM3
• NCBI Official Synonym Symbols: GST5; GSTB; GTM3; GSTM3-3
• NCBI Protein Information: glutathione S-transferase Mu 3
• UniProt Protein Name: Glutathione S-transferase Mu 3
• UniProt Gene Name: GSTM3
• UniProt Synonym Gene Names: GST5; hGSTM3-3