Application Data
(Immunoprecipitation and Western Blot of Herceptin-2 with IP by and WB with "Please inquire". Lane 3,4) IP by and WB by Apparent MW is 174-175 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Phospho-ErbB 2 Positive Control | ErbB 2 positive control
Phospho-ErbB 2 Positive Control
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Applications
Western Blot
Synonyms
Phospho-ErbB 2; N/A; Phospho-ErbB 2 Positive Control; Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; c erb B2/neu protein; CD340; CerbB2; Erb B2; Erythroblastosis Virus; HER 2/neu; Herstatin; Metastatic lymph node gene 19 protein; MLN19; NEU; Neuro Glioblastoma Derived Oncogene Homolog; NGL; p185 ErbB2; Receptor Protein Tyrosine Kinase ErbB2 Precursor; TKR1; Tyrosine kinase-type cell surface receptor HER2; v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian); verbb2 antibody; ErbB 2 positive control
Form/Format
Western Blot Positive Control in SDS sam Ie buffer
Concentration
Empirically set for optimal signal-to-noise ratio (varies by lot)
Sequence Length
1255
Applicable Applications for ErbB 2 positive control
WB (Western Blot)
Immunogen
Purified Protein
Expression
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
Molecular Function
Activator, Kinase, Receptor, Transferase, Tyrosine-protein kinase
Structure
Homodimer. Heterodimer with EGFR, ERBB3 and ERBB4. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with PRKCABP and PLXNB1. Interacts (when phosphorylated on Tyr-1248) with MEMO1. Interacts with MUC1; the interaction is enhanced by heregulin (HRG).
Subcellular Location
Isoform 1: Cell membrane; Single-pass type I membrane protein, Cytoplasm;perinuclear region, Nucleus. Isoform 2: Cytoplasm, Nucleus. Isoform 3: Cytoplasm, Nucleus.
Preparation and Storage
-20 degree C for long term storage
Application Data
(Immunoprecipitation and Western Blot of Herceptin-2 with IP by and WB with "Please inquire". Lane 3,4) IP by and WB by Apparent MW is 174-175 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Application Data
(Immunoprecipitation and Western Blot of Herceptin-2 with IP by and WB with "Please inquire". Lane 3,4) IP by and WB by Apparent MW is 174-175 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Related Product Information for ErbB 2 positive control
Description:
Western Blot Positive Control for Phospho Receptor tyrosine-protein kinase erB-2 (PHER2)
Overview:
The tyrosine kinase receptor erbB2, also known in humans as HER2, is a member of the epidermal growth factor receptor (EGFR or erbB1) family, which also includes erbB3 and erbB4 that are gene products of proto-oncogenes. Protocogenes play an important role in normal proliferation of cells. The interaction of proto-oncogenes with other tumor suppressor proteins (P53, P14ARF etc.) determine the normal or abnormal course of cell cycle and cell division leading to uncontrolled cell proliferation (1). HER2 is found on chromosome 17 and codes for a protein that serves as a cell surface receptor. These HER receptors determine the regulation of cell growth and differentiation in a complex manner. HER family receptors form homo- or hetero- dimers with other compounds. The activation of these homoheterodimmeric complexes by ligand binding leads to activation of intrinsic tyrosine kinase activity that triggers a cascade of signal transduction activity in cell. Some of these signals are transduced across the cell membranes in to cytoplasm and triggers the gene activation. There are at least two humanized recombinant monoclonal antibodies (Trastuzumab and pertuzumab) that target extra cellular regions of the HER2 tyrosine kinase receptor and have been used in human trials for the treatment of breast cancer (2).
Herceptin-2 is a 1342 amino acid protein (approximate MW 170-172 kDa) when analyzed on reduced SOS-PAGE gels. HER2 is over expressed in cells of many cancer types. ErbB receptors bind to a family of growth factors, termed neuregulins/heregulin (NRG/HRG), which comprise NRG-1, -2, -3, and -4 and include multiple variants. HER 2 is a receptor tyrosine kinase with intrinsic tyrosine kinase domain. The Phospho-Her2 antibody (MBS543807) is made against the phospho-Tyrosine containing peptide (aa 1127-1138) from rat HER2 protein.
The PHER2-selective antibodies were generated against unique antigenic sequences form HER2 protein that is not found in any other protein in the gene bank. The Phospho-HER2 antibodies were affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.
Western Blot Positive Control for Phospho Receptor tyrosine-protein kinase erB-2 (PHER2)
Overview:
The tyrosine kinase receptor erbB2, also known in humans as HER2, is a member of the epidermal growth factor receptor (EGFR or erbB1) family, which also includes erbB3 and erbB4 that are gene products of proto-oncogenes. Protocogenes play an important role in normal proliferation of cells. The interaction of proto-oncogenes with other tumor suppressor proteins (P53, P14ARF etc.) determine the normal or abnormal course of cell cycle and cell division leading to uncontrolled cell proliferation (1). HER2 is found on chromosome 17 and codes for a protein that serves as a cell surface receptor. These HER receptors determine the regulation of cell growth and differentiation in a complex manner. HER family receptors form homo- or hetero- dimers with other compounds. The activation of these homoheterodimmeric complexes by ligand binding leads to activation of intrinsic tyrosine kinase activity that triggers a cascade of signal transduction activity in cell. Some of these signals are transduced across the cell membranes in to cytoplasm and triggers the gene activation. There are at least two humanized recombinant monoclonal antibodies (Trastuzumab and pertuzumab) that target extra cellular regions of the HER2 tyrosine kinase receptor and have been used in human trials for the treatment of breast cancer (2).
Herceptin-2 is a 1342 amino acid protein (approximate MW 170-172 kDa) when analyzed on reduced SOS-PAGE gels. HER2 is over expressed in cells of many cancer types. ErbB receptors bind to a family of growth factors, termed neuregulins/heregulin (NRG/HRG), which comprise NRG-1, -2, -3, and -4 and include multiple variants. HER 2 is a receptor tyrosine kinase with intrinsic tyrosine kinase domain. The Phospho-Her2 antibody (MBS543807) is made against the phospho-Tyrosine containing peptide (aa 1127-1138) from rat HER2 protein.
The PHER2-selective antibodies were generated against unique antigenic sequences form HER2 protein that is not found in any other protein in the gene bank. The Phospho-HER2 antibodies were affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
HER2 receptor
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19
UniProt Entry Name
ERBB2_HUMAN
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Product Notes
The ErbB 2 erbb2 (Catalog #AAA75977) is a Positive Control and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phospho-ErbB 2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the ErbB 2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-ErbB 2, Positive Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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