Standard Curve (Sample)
(Human Periostin/OSF2 PicoKine ELISA Kit standard curve)
Human Periostin/OSF2 ELISA Kit | POSTN elisa kit
Human Periostin/OSF2 PicoKine ELISA Kit
Gene Names
POSTN; PN; OSF2; OSF-2; PDLPOSTN; periostin
Reactivity
Human
Synonyms
Periostin/OSF2; N/A; Human Periostin/OSF2 PicoKine ELISA Kit; Periostin; periostin, osteoblast specific factor; Fasciclin-I like; MGC119510; MGC119511; OSF 2; OSF-2; OSF2; Osteoblast specific factor 2 (fasciclin I like); osteoblast specific factor 2 (fasciclin I-like); Osteoblast specific factor 2; Osteoblast specific factor; Osteoblast-specific factor 2; PDLPOSTN; Periodontal ligament specific periostin; Periostin isoform thy2; Periostin isoform thy4; Periostin isoform thy6; Periostin isoform thy8; Periostin osteoblast specific factor; PN; POSTN; POSTN_HUMAN; RP11 412K4.1; POSTN elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human Periostin/OSF2
Sequence Length
836
Samples
Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human Periostin/OSF2 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human Periostin/OSF2 PicoKine ELISA Kit standard curve)
Related Product Information for POSTN elisa kit
Principle of the Assay: Human Periostin/OSF2 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for Periostin/OSF2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: N22-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin/OSF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Periostin/OSF2 amount of sample captured in plate.
Background: Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
Background: Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
80,357 Da
NCBI Official Full Name
Periostin
NCBI Official Synonym Full Names
periostin, osteoblast specific factor
NCBI Official Symbol
POSTN
NCBI Official Synonym Symbols
PN; OSF2; OSF-2; PDLPOSTN; periostin
NCBI Protein Information
periostin; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like)
UniProt Protein Name
Periostin
UniProt Gene Name
POSTN
UniProt Synonym Gene Names
OSF2; PN; OSF-2
UniProt Entry Name
POSTN_HUMAN
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Product Notes
The Human POSTN postn (Catalog #AAA45968) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45968 ELISA Kit recognizes Human POSTN. It is sometimes possible for the material contained within the vial of "Periostin/OSF2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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