Human Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha ELISA Kit | PPARgC1a elisa kit

Human Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha ELISA Kit

Cat. Num. AAA247215

• Gene Names: Ppargc1a; Ppargc1
• Reactivity: Human
• Synonyms: Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha; N/A; Human Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha ELISA Kit; PPARgC1a elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGC1alpha. No significant cross-reactivity or interference between PGC1alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGC1alpha and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 796
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

Standard Curve (Sample)

Related Product Information for PPARgC1a elisa kit

Principle of the Assay: PGC1alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PGC1alpha antibody and an PGC1alpha-HRP conjugate. The assay sample and buffer are incubated together with PGC1alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGC1alpha concentration since PGC1alpha from samples and PGC1alpha-HRP conjugate compete for the anti-PGC1alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by PGC1alpha from the sample, fewer sites are left to bind PGC1alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGC1alpha concentration in each sample is interpolated from this standard curve.

Intended Uses: This PGC1alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PGC1alpha. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 13786188
• NCBI GeneID: 83516
• NCBI Accession #: NP_112637.1
• NCBI GenBank Nucleotide #: NM_031347.1
• UniProt Accession #: Q9QYK2
• Molecular Weight: 90,622 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• NCBI Official Synonym Full Names: PPARG coactivator 1 alpha
• NCBI Official Symbol: Ppargc1a
• NCBI Official Synonym Symbols: Ppargc1
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• UniProt Gene Name: Ppargc1a
• UniProt Synonym Gene Names: Pgc1; Pgc1a; Ppargc1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha