Standard Curve (Sample)
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit | Ppargc1a elisa kit
Rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit
Gene Names
Ppargc1a; Ppargc1
Reactivity
Rat
Synonyms
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; N/A; Rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; Ppargc1a elisa kit
Reactivity
Rat
Specificity
Recombinant and natural Rat PGC-1-alpha
Sequence Length
798
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
0.156-10 ng/mL
Sensitivity
< 0.078 ng/mL
Intra-assay Precision
<=5.9%
Inter-assay Precision
<=8.4%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Ppargc1a elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
90,622 Da
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
NCBI Official Synonym Full Names
PPARG coactivator 1 alpha
NCBI Official Symbol
Ppargc1a
NCBI Official Synonym Symbols
Ppargc1
NCBI Protein Information
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Gene Name
Ppargc1a
UniProt Synonym Gene Names
Pgc1; Pgc1a; Ppargc1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha
UniProt Entry Name
PRGC1_RAT
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Product Notes
The Rat Ppargc1a ppargc1a (Catalog #AAA13302) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA13302 ELISA Kit recognizes Rat Ppargc1a. It is sometimes possible for the material contained within the vial of "Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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