Human Pancreatic prohormone ELISA Kit | PPY elisa kit

Human Pancreatic prohormone ELISA Kit

Cat. Num. AAA17358

• Gene Names: PPY; PP; PNP
• Reactivity: Human
• Synonyms: Pancreatic prohormone; Human Pancreatic prohormone ELISA Kit; PPY/INN/Obinepitide/PNP/PP/Obinepitide/pancreatic polypeptide (ppy)/pancreatic polypeptide/pancreatic polypeptide Y/pancreatic prohormone; PPY elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGF2alpha. No significant cross-reactivity or interference between PGF2alpha and analogues is observed.
• Sequence Length: 95
• Assay Type: Competitive ELISA, Coated with Antigen
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 46.875-3000pg/ml
• Sensitivity: 28.125pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PGF2alpha were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PGF2alpha were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for PPY elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with PGF2alpha. During the reaction, PGF2alpha in the sample or standard competes with a fixed amount of PGF2alpha on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PGF2alpha. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PGF2alpha in the samples is then determined by comparing the O D of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506033
• NCBI GeneID: 5539
• NCBI Accession #: NP_002713.1
• NCBI GenBank Nucleotide #: NM_002722.4
• Molecular Weight: 18,786 Da
• NCBI Official Full Name: pancreatic prohormone isoform 1 preproprotein
• NCBI Official Synonym Full Names: pancreatic polypeptide
• NCBI Official Symbol: PPY
• NCBI Official Synonym Symbols: PP; PNP
• NCBI Protein Information: pancreatic prohormone
• UniProt Protein Name: Pancreatic prohormone
• UniProt Gene Name: PPY
• UniProt Synonym Gene Names: PNP; PP; PH; PI
• UniProt Entry Name: PAHO_HUMAN