Standard Curve (Sample)
General Prolactin ELISA Kit | PRL elisa kit
Rat Prolactin ELISA Kit
Gene Names
Prl; PRLB; Prol; PRLSD1; Prl1a1; RNPROL; RATPRLSD1
Reactivity
General
Synonyms
Prolactin; N/A; Rat Prolactin ELISA Kit; PRL elisa kit
Reactivity
General
Specificity
Pooled normal plasma from pig, dog, human and sheep was assayed and no cross-reactivity was observed. Pooled normal mouse and horse plasma showed significant cross-reactivity. Pooled normal plasma from rhesus monkey, cyno monkey and rabbit resulted in significant background color development.
Form/Format
Complete Kit
Sequence Length
226
Sensitivity
0.36 ng/mL
Intra-assay Precision
These studies are currently in progress. Please contact us for more information.
Inter-assay Precision
These studies are currently in progress. Please contact us for more information.
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PRL elisa kit
Background: Rat prolactin (PRL) is a 197 aa, 23kD peptide hormone [1] that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation [2,3]. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk [4].
Principle of the Assay: Rat prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, anti-rat prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat prolactin. Color development is proportional to the concentration of prolactin in the samples.
Intended Uses: This rat prolactin antigen assay is intended for the quantitative determination of prolactin antigen in rat plasma.
Principle of the Assay: Rat prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, anti-rat prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat prolactin. Color development is proportional to the concentration of prolactin in the samples.
Intended Uses: This rat prolactin antigen assay is intended for the quantitative determination of prolactin antigen in rat plasma.
Product Categories/Family for PRL elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,753 Da
NCBI Official Full Name
prolactin
NCBI Official Synonym Full Names
prolactin
NCBI Official Symbol
Prl
NCBI Official Synonym Symbols
PRLB; Prol; PRLSD1; Prl1a1; RNPROL; RATPRLSD1
NCBI Protein Information
prolactin; prolactin family 1, subfamily a, member 1
UniProt Protein Name
Prolactin
UniProt Gene Name
Prl
UniProt Synonym Gene Names
PRL
UniProt Entry Name
PRL_RAT
Similar Products
Product Notes
The General PRL prl (Catalog #AAA37733) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA37733 ELISA Kit recognizes General PRL. It is sometimes possible for the material contained within the vial of "Prolactin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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