Standard Curve (Sample)
Mouse prolactin ELISA Kit | PRL elisa kit
Mouse Prolactin/Luteotropic Hormone, PRL/LTH ELISA Kit
Gene Names
Prl; Prl1a1; AV290867
Reactivity
Mouse
Synonyms
prolactin; N/A; Mouse Prolactin/Luteotropic Hormone, PRL/LTH ELISA Kit; Mouse Prolactin/Luteotropic Hormone (PRL/LTH) ELISA Kit; PRL elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse PRL. No significant cross-reactivity or interference between Mouse PRL and analogues was observed.
Sequence Length
226
Samples
Serum, plasma, tissue homogenates
Detection Range
0.06 ng/ml-25 ng/ml
Sensitivity
0.06 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
fertilization
Protein Biological Process 3
Lactation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PRL elisa kit
Introduction: Prolactin (PRL) or Luteotropic hormone (LTH) is a peptide hormone primarily associated with lactation. PRL is synthesised and secreted by lactotrope cells in the adenohypophysis. It is also produced in other tissues including the breast, the decidua, parts of the central nervous system and the immune system. Pituitary prolactin secretion is regulated by neuroendocrine neurons in the hypothalamus, the most important ones being the neurosecretory tuberoinfundibulum (TIDA) neurons of the arcuate nucleus, which secrete dopamine to act on the dopamine-2 receptors of lactotrophs, causing inhibition of prolactin secretion. Thyrotropin-releasing factor has a stimulatory effect on prolactin release. Mouse prolactin is a single-chain polypeptide hormone containing 197 amino acid residues with a molecular weight of 23 kDa. In the mouse, there is a large family of paralogous genes closely related to PRL. PRL family genes reside on chromosome 13 of the mouse genome.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated PRL and anti-PRL antibody and incubated. Then substrate solution A and B are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PRL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated PRL and anti-PRL antibody and incubated. Then substrate solution A and B are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PRL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,496 Da
NCBI Official Full Name
Prolactin
NCBI Official Synonym Full Names
prolactin
NCBI Official Symbol
Prl
NCBI Official Synonym Symbols
Prl1a1; AV290867
NCBI Protein Information
prolactin
UniProt Protein Name
Prolactin
UniProt Gene Name
Prl
UniProt Synonym Gene Names
PRL
UniProt Entry Name
PRL_MOUSE
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Product Notes
The Mouse PRL prl (Catalog #AAA79567) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79567 ELISA Kit recognizes Mouse PRL. It is sometimes possible for the material contained within the vial of "prolactin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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