Standard Curve (Sample)
Human Major prion protein ELISA Kit | PRNP elisa kit
Human Major prion protein ELISA Kit
Gene Names
PRNP; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
Reactivity
Human
Synonyms
Major prion protein; Human Major prion protein ELISA Kit; PRNP/PrP33-35C/ASCR/PrP27-30/CJD/GSS/PrP/ASCR/KURU/PRIP/PrPc/CD230/AltPrP/p27-30/PrP33-35C; PRNP elisa kit
Reactivity
Human
Specificity
Specifically recognize PRNP, no obvious cross reaction with other analogues
Sequence Length
253
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PRNP elisa kit
Background: The prion protein (PRNP) is a glycoprotein that exists in membrane-bound and non-membrane-bound forms. PRNP binds copper ion with low affinity and may affect copper metabolism, especially in the central nervous system, where this protein is highly expressed. The interaction between copper and PRNP may have a protective effect on neurons. Diseases associated with PRNP include Creutzfeldt-Jakob Disease and Fatal Familial Insomnia. Among its related pathways are Nervous system development and NCAM signaling for neurite out-growth.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti PRNP antibody was pre-coated onto the 96-well plate. The biotin conjugated anti PRNP antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with PRNP conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of PRNP in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti PRNP antibody was pre-coated onto the 96-well plate. The biotin conjugated anti PRNP antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with PRNP conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of PRNP in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8,691 Da
NCBI Official Full Name
major prion protein preproprotein
NCBI Official Synonym Full Names
prion protein
NCBI Official Symbol
PRNP
NCBI Official Synonym Symbols
CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
NCBI Protein Information
alternative prion protein; major prion protein
UniProt Protein Name
Major prion protein
UniProt Gene Name
PRNP
UniProt Synonym Gene Names
ALTPRP; PRIP; PRP; PrP
UniProt Entry Name
PRIO_HUMAN
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Product Notes
The Human PRNP prnp (Catalog #AAA17478) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17478 ELISA Kit recognizes Human PRNP. It is sometimes possible for the material contained within the vial of "Major prion protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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